| 颗粒状脱细胞真皮基质的形态学特征及理化性能体外研究 |
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| 引用本文： | 左海斌,彭代智,郑必祥,何升东,周新,刘敬. 颗粒状脱细胞真皮基质的形态学特征及理化性能体外研究[J]. 第三军医大学学报, 2011, 33(1): 4-8 |
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| 作者姓名： | 左海斌 彭代智 郑必祥 何升东 周新 刘敬 |
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| 作者单位： | 第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆,400038 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，国家重点基础研究发展计划(973计划)项目 |
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| 摘 要： | 目的 研究颗粒状脱细胞真皮基质(particulate acellular dermal matrix,PADM)的体外形态学特征与理化性能.方法 收集SD大鼠背部皮肤样本,采用本实验室的脱细胞方法制备片状脱细胞真皮基质(acellular dermal matrix,ADM)并分析其内胞核和DNA残留情况.将片状AD...
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| 关 键 词： | 细胞外基质 颗粒状脱细胞真皮基质 粒径分布 傅里叶变换红外光谱仪 |
In vitro investigation on morphological and physico-chemical properties of particulate acellular dermal matrix |
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| ZUO Haibin,Peng Daizhi,Zheng Bixiang,He Shengdong,Zhou Xin,Liu Jing. In vitro investigation on morphological and physico-chemical properties of particulate acellular dermal matrix[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(1): 4-8 |
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| Authors: | ZUO Haibin Peng Daizhi Zheng Bixiang He Shengdong Zhou Xin Liu Jing |
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| Affiliation: | Zuo Haibin,Peng Daizhi,Zheng Bixiang,He Shengdong,Zhou Xin,Liu Jing(State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burn Research,Southwest Hospital,Third Military Medical University,Chongqing,400038,China) |
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| Abstract: | Objective To investigate the morphological and physico-chemical properties of particulate acellular dermal matrix(PADM) in vitro.Methods Dorsal skin samples were collected from SD rats,and then were decellularized to obtain acellular dermal matrix(ADM) sheets.The ADM sheets were cut into PADM in different sizes.Digital camera,light microscope(HE staining) and electron microscopes were used to observe the morphological properties of PADM.Laser diffraction and Fourier transform infrared spectroscopy(FTIR) wer... |
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| Keywords: | extracellular matrix particulate acellular dermal matrix size distribution fourier transform infrared spectroscopy |
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