| Roles of Chemokine Receptor 4 （CXCR4） and Chemokine Ligand 12 （CXCL12） in Metastasis of Hepatocellular Carcinoma Cells |
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| 引用本文： | Liu H,Pan Z,Li A,Fu S,Lei Y,Sun H,Wu M,Zhou W. Roles of Chemokine Receptor 4 （CXCR4） and Chemokine Ligand 12 （CXCL12） in Metastasis of Hepatocellular Carcinoma Cells[J]. Cellular & molecular immunology, 2008, 5(5): 373-378 |
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| 作者姓名： | Liu H Pan Z Li A Fu S Lei Y Sun H Wu M Zhou W |
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| 作者单位： | The 3rd Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China |
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| 摘 要： | Chemokines are involved in human hepatocellular carcinoma （HCC） carcinogenesis. However, the exact mechanism of chemokines in HCC carcinogenesis remains unknown. Here we investigated the roles of chemokine receptor 4 （CXCR4） and chemokine ligand 12 （CXCL12） in the metastasis of HCC. We found that the expression levels of CXCR4 mRNA in HCC tissues, MHCC97 cells, and HUVEC cells were 2.52 ±1.13, 2.34 ±1.16 and 1.63 ±1.26, respectively and that the CXCR4 protein levels were 1.38 ± 0.13, 1.96± 0.32 and 1.86 ±0.21, respectively. In contrast, CXCR4 was not detected in normal hepatic tissues. In 78 HCC patients, we also found that the concentration of CXCL12 in cancerous ascitic fluid was 783-8,364 pg/ml and that CXCL12 mRNA level in HCC metastasis portal lymph nodes was 1.21 ± 0.87 but undetectable in normal hepatic tissues. Finally we discovered that recombinant human CXCL12 could induce MHCC97 cells and HUVEC cells to migrate with chemotactic indexes （CI） of 3.9 ±1.1 and 4.1± 1.6, respectively. Cancerous ascitic fluid could also induce the migration of MHCC97 cells with a CI of 1.9 ± 0.8. Thus, our data suggest that CXCR4 and CXCL12 may play an important role in the metastasis of HCC by promoting the migration of tumor cells.
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| 关 键 词： | 肝细胞癌 肝移植 肿瘤转移 CXCR4 CXCL12 |
Roles of chemokine receptor 4 (CXCR4) and chemokine ligand 12 (CXCL12) in metastasis of hepatocellular carcinoma cells |
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| Liu Hui,Pan Zeya,Li Aijun,Fu Siyuan,Lei Yin,Sun Hangyong,Wu Mengchao,Zhou Weiping. Roles of chemokine receptor 4 (CXCR4) and chemokine ligand 12 (CXCL12) in metastasis of hepatocellular carcinoma cells[J]. Cellular & molecular immunology, 2008, 5(5): 373-378 |
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| Authors: | Liu Hui Pan Zeya Li Aijun Fu Siyuan Lei Yin Sun Hangyong Wu Mengchao Zhou Weiping |
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| Affiliation: | Hui Liu1,Zeya Pan1,Aijun Li1,Siyuan Fu1,Yin Lei1,Hangyong Sun1,Mengchao Wu1 , Weiping Zhou1,21The 3rd Department of Hepatic Surgery,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Shanghai,200438,China |
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| Abstract: | Chemokines are involved in human hepatocellular carcinoma (HCC) carcinogenesis. However, the exact mechanism of chemokines in HCC carcinogenesis remains unknown. Here we investigated the roles of chemokine receptor 4 (CXCR4) and chemokine ligand 12 (CXCL12) in the metastasis of HCC. We found that the expression levels of CXCR4 mRNA in HCC tissues, MHCC97 cells, and HUVEC cells were 2.52 +/- 1.13, 2.34 +/- 1.16 and 1.63 +/- 1.26, respectively and that the CXCR4 protein levels were 1.38 +/- 0.13, 1.96 +/- 0.32 and 1.86 +/- 0.21, respectively. In contrast, CXCR4 was not detected in normal hepatic tissues. In 78 HCC patients, we also found that the concentration of CXCL12 in cancerous ascitic fluid was 783-8,364 pg/ml and that CXCL12 mRNA level in HCC metastasis portal lymph nodes was 1.21 +/- 0.87 but undetectable in normal hepatic tissues. Finally we discovered that recombinant human CXCL12 could induce MHCC97 cells and HUVEC cells to migrate with chemotactic indexes (CI) of 3.9 +/- 1.1 and 4.1 +/- 1.6, respectively. Cancerous ascitic fluid could also induce the migration of MHCC97 cells with a CI of 1.9 +/- 0.8. Thus, our data suggest that CXCR4 and CXCL12 may play an important role in the metastasis of HCC by promoting the migration of tumor cells. |
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| Keywords: | CXCR4 CXCL12 hepatocellular carcinoma migration metastasis |
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