室温下光线照射对血清胆红素测定结果的影响

目的探讨室温下血清标本在实验室放置不同时间和受实验室光线照射对总胆红素和直接胆红素测定结果的影响。方法将一组标本暴露在实验室光线下,另一组标本避光保存,分别于0、2、4、8、24h测定总胆红素和直接胆红素,对比分析两组标本测定结果的平均偏差。结果室温下血清标本在0h测定的总胆红素结果为（17.47±9.33）μmol/L,2、4、8、24h后光照组总胆红素结果为（16.73±9.09）、（15.98±8.85）、（15.13±8.35）、（13.50±7.37）μmol/L（P〈0.05）;2、4、8、24h后避光组总胆红素结果为（17.47±9.45）、（17.58±9.60）μmol/L（P〉0.05）、（17.92±9.95）、（19.86±11.21）μmol/L（P〈0.01）;室温下血清标本在0h测定的直接胆红素结果为（5.40±3.19）μmol/L,2、4、8、24h后光照组直接胆红素结果为（5.49±3.22）μmol/L（P〉0.05）、（5.55±3.26）、（5.80±3.37）、（6.08±3.64）μmol/L（P〈0.05）;2、4、8、24h后避光组总胆红素结果为（5.28±3.17）、（5.39±3.28）、（5.51±3.40）、（5.63±3.72）μmol/L（P〉0.05）。结论室温下受光线照射血清标本的总胆红素不断下降,2h后总胆红素测定值差异也有统计学意义,避光保存组的血清总胆红素标本测定值略微上升,但至少可以保存4h;室温下受光线照射血清标本的直接胆红素值不断上升,但2h内检测结果差异无统计学意义,避光组的直接胆红素标本测定值也不断上升,但至少可以保存24h。

The effect of laboratory light ray at room temperature on the result of determination of serum bilirubin

Objective To explore effects of serum samples at room temperature in different time and a light ray laboratory on results of total bilirubin and direct bilirubin determination.Methods A group in the laboratory specimen were exposed in light,another group of specimens were preserved avoiding light,respectively.Total bilirubin and direct bilirubin were detected in 0,2,4,8,24 h,comparative analysis of the two groups of specimens of the average deviation determination was taken.Results Serum samples at room temperature in 0 h determination of the total bilirubin result was（17.47 ±9.33）μmol/L.2,4,8,24 h after light,total bilirubin results were（16.73 ±9.09）,（15.98 ±8.85）,（15.13 ±8.35）,（3.50 ±7.37）μmol/L（P0.05）.After 2,4,8,24 h avoiding light,determination of the total bilirubin results were（17.47 ±9.45）,（17.58 ±9.60）μmol/L（P0.05）,（17.92 ±9.95）,（19.86±11.21）μmol/L（P0.01）.Serum samples at room temperature in 0 h determination of the direct bilirubin result was（5.40 ±3.19）μ mol/L.After 2,4,8,24 h light,determination of direct bilirubin the results were（5.49±3.22）（P0.05）,（5.55±3.26）,（5.80±3.37）,（6.08±3.64）（P0.05）μmol/L.After 2,4,8,24 havoiding light,determination of the total bilirubin results were（5.28±3.17）,（5.39±3.28）,（5.51±3.40）,（5.63±3.72）μmol/L（P0.05）.Conclusion Serum samples of total bilirubin values falling,total bilirubin determination value has statistical significance after 2 h in light ray at room temperature.avoiding light preservation can save the serum total bilirubin specimens determination value with rising slightly,but these can be stored for at least 4 h.Room temperature with a light ray,direct bilirubin serum blood samples values are rising,but after 2 h results have no statistical significance,avoid light group′s direct bilirubin specimens are rising,but these can be stored for at least 24 h.