外阴鳞状细胞癌组织中VEGF和Bcl-2基因组蛋白H3K4三甲基化水平的表达

目的：检测外阴鳞状细胞癌患者癌组织血管内皮生长因子（VEGF）和Bcl-2基因组蛋白H3K4三甲基化（me3）甲基状态及其mRNA的表达。方法：采用染色质免疫共沉淀一实时定量聚合酶链反应（ChIP—qPCR）技术对15例外阴鳞状细胞癌患者癌组织和毗邻正常组织VEGF和Bcl-2基因组蛋白H3K4me3甲基化状态进行定量分析；随后采用定量反转录聚合酶链反应（qRT—PCR）检测基因的mRNA表达。结果：外阴鳞状细胞癌组织中VEGF和Bcl-2基因呈现组蛋白H3K4me3水平增高（P〈0．05）：VEGF和Bcl-2基因的mRNA表达增加。结论：外阴鳞状细胞癌组织VEGF和Bcl-2基因可能参与外阴鳞状细胞癌的发生，成为外阴鳞状细胞癌新的治疗靶点。

The expression of histone H3 lysine 4 trimethylation of VEGF and BCL - 2 gene in vulvar squamous cell carcinoma tissue

Objective： To detect the expression of the histone H3 lysine 4 trimethylation （H3K4me3） of the VEGF and Bcl- 2 gene in vulvar squamous cell carcinoma （VSCC）. Methods： Chromatin immuno- precipitation - quantitative PCR was used to detect the methylation level of VEGF and Bcl - 2 gene in 15 patients with VSCC and the control tissues. The mRNA expression on the VEGF and Bcl - 2 gene was performed by qRT - PCR. Results： Compared to normal tissues, The H3K4me3 of VEGF and Bcl - 2 gene in VSCC increased （ P 〈 0.05）. The expression of mRNA of VEGF and Bcl - 2 gene also increased. Conclusions： The VEGF and Bcl - 2 gene may be in- volved in the pathogenesis of VSCC, which may become a novel potential biomarker for the treatment of VSCC.