| 氧化苦参碱对HepG2.2.15细胞中乙型肝炎病毒DNA表达量的影响 |
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| 引用本文： | 徐文胜,王国俊,缪晓辉,蔡雄.氧化苦参碱对HepG2.2.15细胞中乙型肝炎病毒DNA表达量的影响[J].第二军医大学学报,2002,23(1):72-73. |
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| 作者姓名： | 徐文胜 王国俊 缪晓辉 蔡雄 |
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| 作者单位： | 第二军医大学长征医院感染科,上海,200003 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 0 70 95 8) |
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| 摘 要： | 目的:观察氧化苦参碱对HepG2.2.15细胞中乙型肝炎病毒(HBV) DNA表达量的影响,进一步探讨其抗病毒机制.方法:用不同浓度的氧化苦参碱作用HepG2.2.15细胞,留取培养上清后,采用PCR-ELISA技术定量上清中HBV DNA,并比较其抑制率.结果:氧化苦参碱能直接抑制HepG2.2.15细胞内HBV DNA的复制,且随着药物浓度升高抑制作用逐渐增强.保持药物在培养上清中的浓度是保证抑制率的关键.结论:氧化苦参碱可以在DNA复制水平直接抑制乙型肝炎病毒的合成.
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| 关 键 词： | 氧化苦参碱、肝炎病毒 乙型、DNA病毒 |
| 文章编号： | 0258-879X(2002)01-0072-02 |
| 修稿时间： | 2001年6月16日 |
Effects of oxymatrine on expression of HBV DNA in HepG2.2.15 cells |
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| XU Wen-Sheng,WANG Guo-Jun,MIAO Xiao-Hui,CAI Xiong.Effects of oxymatrine on expression of HBV DNA in HepG2.2.15 cells[J].Academic Journal of Second Military Medical University,2002,23(1):72-73. |
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| Authors: | XU Wen-Sheng WANG Guo-Jun MIAO Xiao-Hui CAI Xiong |
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| Abstract: | Objective: To observe the effect of oxymatrine (OM) on the expression of HBV DNA in HepG2.2.15 cells and to investigate the anti-HBV mechanism of OM. Methods: The level of HBV DNA in HepG2.2.15 cells incubated with different concentrations of OM was quantified by equivalent competitive PCR combining with DNA hybridization quantitative detection technique (PCR-ELISA). The in vitro anti-viral effect of OM was evaluated by calculating the inhibiting rate. Results: OM inhibited the expression of HBV in HepG2.2.15 cells. The inhibiting rate increased with the drug concentration. The stable concentration of OM in medium was important in keeping the inhibiting rate. Conclusion: OM can inhibit the synthesis of HBV directly at the level of HBV DNA replication. |
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| Keywords: | oxymatrine hepatitis B virus DNA viral |
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