| 高效液相色谱法测定小青龙合剂中盐酸麻黄碱含量 |
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| 引用本文： | 周颖,刘哨凡,霍晓梅. 高效液相色谱法测定小青龙合剂中盐酸麻黄碱含量[J]. 中国药业, 2007, 16(20): 37-38 |
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| 作者姓名： | 周颖 刘哨凡 霍晓梅 |
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| 作者单位： | 1. 广西壮族自治区玉林食品药品检验所,广西,玉林,537000
2. 桂林医学院药学院,广西,桂林,541001 |
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| 摘 要： | 目的建立小青龙合剂中盐酸麻黄碱的含量测定方法。方法色谱柱为Hypersil ODS柱（250mm×4．6mm，5μm），0.02mol／L磷酸二氢钾溶液（含0.2％三乙胺，用磷酸调节pH值至2．7）-乙腈（97.5：2.5）为流动相，流速为1.0mL／min，检测波长为210nm。结果盐酸麻黄碱质量浓度在14．16-354μg／mL范围内与峰面积呈良好的线性关系，r=0．9999，平均回收率为98．8％，RSD=1．75％（n=9）。结论该方法简便，结果准确，重现性、专属性好，适用于该制剂的定量分析。
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| 关 键 词： | 高效液相色谱法 盐酸麻黄碱 小青龙合剂 |
| 文章编号： | 1006-4931（2007）20-0037-02 |
| 修稿时间： | 2007-01-21 |
Determination of Ephedrine Hydrochloride in Xiaoqinglong Mixture by HPLC |
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| Zhou Ying,Liu Shaofan,Huo Xiaomei. Determination of Ephedrine Hydrochloride in Xiaoqinglong Mixture by HPLC[J]. China Pharmaceuticals, 2007, 16(20): 37-38 |
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| Authors: | Zhou Ying Liu Shaofan Huo Xiaomei |
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| Affiliation: | 1. Guangxi Yulin Institute For Food and Drug Control, Yulin, Guangxi, China 537000; 2. Department of Pharmacy, Guilin Medical College, Guilin, Guangxi, China 541001 |
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| Abstract: | Objective To establish HPLC method for the determination of ephedrine hydrochloride in Xiaoqinglong Mixture. Methods The operating condition were Hypersil ODS （250 mm × 4.6 mm, 5 μm）, 0.02 mol/L potassium dihydrogen phosphoric （containing 0, 2% triethaneamine, the pH was adjusted to 2.7 by phosphoric acid）-acetonitrile（97.5：2.5）,The flow rate was 1.0 mL/min, and the UV detective wavelength was set at 210 nm, Results The linear range of the calibration curve of ephedrine hydrochloride was 14.16-354 μg/mL with a correlation coefficient of 0.999 9. The average recovery rate was 98.8% , RSD was 1.75% （ n = 9 ）. Conclusion The method is simple, accurate and reproducible, It can be used for the determination of ephedrine hydrochloride in Xiaoqinglong Mixture. |
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| Keywords: | HPLC ephedrine hydrochloride Xiaoqinglong Mixture |
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