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| 明党参愈伤组织诱导及植株再生 | |
| 引用本文： | 徐秀泉,严春艳,于荣敏.明党参愈伤组织诱导及植株再生[J].中药材,2005,28(11):971-973. |
| 作者姓名： | 徐秀泉 严春艳 于荣敏 |
| 作者单位： | 1. 江苏大学生物与环境工程学院,江苏镇江212013 2. 中国药科大学中药学院,江苏南京210009 3. 暨南大学药学院,广东广州510632 |
| 摘 要： | 目的:通过愈伤组织的增殖与分化实现快速繁殖,以建立明党参愈伤组织培养体系.方法:以明党参的嫩茎为外植体,在不同激素组合的脱分化、分化培养基上培养.结果:MS培养基附加NAA 0.5～1 mg/L,KT 0.1 mg/L适合于愈伤组织的诱导和培养;附加6-BA 1～2 mg/L适合愈伤组织芽的分化;附加IBA 0.5～1 mg/L适合根的诱导.结论:在一定培养条件下,通过愈伤组织的再分化是实现明党参植株再生的有效途径. |
| 关 键 词： | 明党参 愈伤组织 再分化 植株再生 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005年4月11日 |
Plant Regeneration from Callus of Changiums myrnioides |
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| Xu Xiuquan,Yan Chunyan,Yu Rongmin.Plant Regeneration from Callus of Changiums myrnioides[J].Jorunal of Chinese Medicinal Materials,2005,28(11):971-973. | |
| Authors: | Xu Xiuquan Yan Chunyan Yu Rongmin |
| Institution: | 1. College of Biological and Environmental Science, Jiangsu University, Zhenjiang 212013; 2. College of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 210009 ; 3. College of Pharmacy, Jinan University, Guangzhou 510632 |
| Abstract: | OBJECTIVE: To set up the callus culture system of Changiums myrnioides and the plantlet regeneration system by the proliferation and differentiation of callus. METHOD: The segment of stems used as the explants were cultured on dedifferentiation and differentiation medium supplied with different auxin and cytokinin. RESULT: MS medium with NAA 0.5 - 1.0 mg/L + KT 0.1 mg/L was best combination for the induction of callus, MS medium with 6 - BA 1 - 2 mg/L + GA 1 - 2 mg/L was suitable for the differentiation of adventitious buds, and MS medium with IBA 0.5 - 1 mg/L was suitable for the induction of root. CONCLUSION: Callus culture may be used as a useful method to get regenerated plantlet of Changiums myrnioides under certain condition. |
| Keywords: | Changiums myrnioides Callus culture Dedifferentiation Plantlet regeneration |
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