EFFECT OF TEMPERATURE UPON THE CIRCULAR DICHROISM OF BRADYKININ |
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Authors: | JOHN R CANN ROBERT E LONDON JOHN M STEWART NICHOLAS A MATWIYOFF |
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Abstract: | Analysis of the effect of temperature on the circular dichroism spectrum of bradykinin has led to a more precise understanding of the solution conformation of the peptide. Circular dichroism and 13C n.m.r. have been used in a complementary fashion to support the picture that bradykinin spends a maximum of about 20% of its time in a partially ordered conformation featuring a γ-turn with Pro7 as the second residue. Since the γ-turn probability is insensitive to temperature, some other conformational effect dominated by the structure of water presumably produces the pronounced change in the circular dichroism spectrum with increasing temperature. |
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Keywords: | bradykinin circular dichroism 13 Cn m r peptide conformation |
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