ANTIBODIES AGAINST HAPTENS IN THE STUDY OF STRUCTURE AND BIOLOGICAL ACTIVITY OF GROWTH HORMONE

The α-amino group of bovine Growth Hormone was selectively modified with TNBS with no detectable changes in growth promoting activity. TNP was used as hapten for the production of antibodies against the end terminal region of bGH. The region near the α-amino group is not involved in the binding of bGH to rat liver cells, and it seems to be away from the part of the molecule that interacts with the cell binding sites.