参茸五子丸的急性毒性试验研究

目的:对参茸五子丸进行急性毒性试验,评价参茸五子丸的安全性,为临床应用提供依据。方法:40只小鼠,每组20只,随机分为2组,标记为参茸五子丸组和空白组,试验前禁食不禁水12 h,参茸五子丸组按40.0 m L·kg^-1灌服参茸五子丸溶液(1.15 g·m L^-1),每日2次,空白组小鼠等体积同方法纯水灌服。给药后,观察两周,对比小鼠是否有中毒反应并记录小鼠死亡数目。结果:观察期间动物生长正常,未见任何中毒现象,未见死亡;14 d后处死小鼠,各器官均正常。结论:小鼠的最大给药量为92.0 g生药/kg。小鼠给予参茸五子丸灌胃的最大耐受剂量大于92.0 g生药/kg。

Study on Acute Toxicity Test of Shenrong Wuzi Pill

Objective Acute toxicity test was performed on Shenrong Wuzi Pills to evaluate the safety of Shenrong Wuzi Pills and to provide a basis for clinical application.Methods 40 mice,20 in each group,were randomly divided into two groups,marked as Shenrong Wuzi pill group and blank group.Before the test,the mice in Shenrong Wuzi pill group were fasted but not water for 12 hours.The mice in Shenrong Wuzi pill group were given 40.0 mL kg^-1 solution(1.15 g m L^-1)twice a day,and the mice in blank group were given the same volume of pure water.After administration,the mice were observed for two weeks to compare whether there was toxic reaction and record the number of mouse deaths.during the observation period,the animals grew normally without any poisoning or death.After 14 days,all organs were normal.Results During the observation period,the animals grew normally without any poisoning or death;after 14 days,all organs were normal.Conclusion The maximum dose for mice was 92.0 g crude drug/kg.The maximum tolerated dose of mice given Shenrong Wuzi Wan gavage is greater than 92.0 g crude drug/kg.