| 大鼠肠神经系统乙酰胆碱酯酶和抗氟化物酸性磷酸酶双染法研究 |
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| 引用本文： | 雷亚宁,胡道松,茹立强.大鼠肠神经系统乙酰胆碱酯酶和抗氟化物酸性磷酸酶双染法研究[J].解剖学杂志,1995(3). |
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| 作者姓名： | 雷亚宁 胡道松 茹立强 |
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| 作者单位： | 同济医科大学神经生物学教研室!武汉430030 |
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| 摘 要： | 用抗氟化物酸性磷酸酶(FRAP)作为感觉神经元的化学标志物,同时结合乙酰胆碱酯酶组织化学方法,对大鼠小肠壁神经结构进行了组织化学观察.结果发现了三种不同染色的神经元:(1)乙酰胆碱酯酶阳性神经元;(2)抗氟化物酸性磷酸酶单染神经元;(3)乙酰胆碱酯酶一抗氟化物酸性磷酸酶双染神经元.这提示大鼠肠神经系统内不仅存在有感觉神经元,而且,部分感觉神经元可能是胆碱能性质的.
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| 关 键 词： | 小肠 神经元 大鼠 |
A STUDY ON DOUBLE-STAINING METHOD OF ACETYLCHOLINESTERASE AND FLUORIDE-RESISTANT ACID PHOSPHATASE ON ENTERIC NERVOUS SYSTEM OF RAT |
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| Lei Yaning,Hu Daosong,Ru Liqiang.A STUDY ON DOUBLE-STAINING METHOD OF ACETYLCHOLINESTERASE AND FLUORIDE-RESISTANT ACID PHOSPHATASE ON ENTERIC NERVOUS SYSTEM OF RAT[J].Chinese Journal of Anatomy,1995(3). |
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| Authors: | Lei Yaning Hu Daosong Ru Liqiang |
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| Abstract: | Histochemica) observations on the enteric nervous system (ENS) of the rats were made by using fluoride-resistant acid phosphatase (FRAP) as a chemical marker for sensory neurons combined with acetylcholinesterase (AChE) histochemical method. Three different types of stained neurons, AChE positive, FRAP single stained, and AChE-FRAP double stained, were found. The results suggest that there are sensory nensory neurons in ENS of the rats, some of which may be cholinergic. |
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| Keywords: | small intestine: neuron rat |
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