糖基化终产物促进培养的人脐静脉内皮细胞选择素E的表达

目的 探讨糖基化终产物在糖尿病动脉粥样硬化形成中的作用机理。方法 分离正常人脐静脉内皮细胞，将糖基化终产物修饰的人血清白蛋白及未修饰人血清白蛋白与人脐静脉内皮细胞在体外共同培养，用荧光单克隆抗体染色，流式细胞仪定量检测内皮细胞表面选择素E的表达。结果 正常人脐静脉内皮细胞未表达选择素Eo糖基化终产物修饰的人血清白蛋白能以时间和剂量依赖的方式上调血管内皮细胞粘附分子选择素E的表达（P〈0．015），而人血清白蛋白对人脐静脉内皮细胞粘附分子选择素E的表达无影响。结论 糖基化终产物能上调人脐静脉内皮细胞粘附分子的表达，从而促进动脉粥样硬化时单核，巨噬细胞的浸润。

Advanced Glycation End Products Modified Protein Up-regulate Expression of Adhesion Molecules E-Selectin on Human Umbilical Vein Endothelial Cell

Aim Progressive vascular disease is the leading cause of death in patient with diabetes mellitus. Advanced glycation end products (AGE) modified proteins are found in plasma and atherosclerosis lesion of diabetes mellitus patients. The study was conducted to elucidate the infection of AGE on expression of adhesion molecules E-selectin on human endothelial cell. Methods Human umbilical vein endothelial cell were coincubated in vitro with native human serum albumin(HSA), HSA modified with advanced glycation end products(AGE-HSA). The expression of adhesion molecule E-selectin was determined by immunofluorescen staining and flow cytometer analysis. Results E-selectin was not constitutively expressed on hUVEC. AGE-HSA enhanced the express of E-selectin on hUVEC in a time- and dose- dependent manner. HSA had no effect on the expression of adhesion molecule. Conclusion AGE up-regulates the expression of adhesion molecule on human endothelial cell and this can promote the infiltration of monocytes/macrophages in the intima and the formation of atherosclerosis.