1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙胺基)丙烷盐酸盐对自发性高血压大鼠血管平滑肌细胞增殖及对PDGF-B,bFGF,c-sis,c-myc的影响

用氚 胸腺嘧啶核苷(³H-TdR)掺入法,电镜,免疫组化,原位杂交方法,在自发性高血压大鼠(SHR)观察了1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙胺基)丙烷盐酸盐(DDPH)对血管平滑肌细胞(VSMC)增殖的作用及对生长因子PDGF-B,bFGF及其相关癌基因c-sis与c-myc表达的影响。结果发现:DDPH在降低SHR血压同时,能减少肾动脉VSMC的线粒体,粗面内质网和³H-TdR掺入量,并能逆转VSMC增殖时PDGF-B,bFGF抗原及c-sis与c-mycmRNA的表达增强。提示:DDPH能抑制SHR的VSMC增殖,与生长因子及癌基因调控的分子生物学机制有关。

Effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethylamino) propane hydrochloride on proliferation of vascular smooth muscle cells and PDGF-B, bFGF, c-sis, c-myc in spontaneously hypertensive rat]

In order to determine the effects of 1-(2, 6-dimethylphenoxy)-2-(3, 4-dimethoxyphenylethylamino) propane hydrochloride(DDPH) on proliferation of vascular smooth muscle cells(VSMC), growth factor PDGF-B, bFGF and expression of oncogene c-sis and c-myc in spontaneously hypertensive rat(SHR), 3H-thymidine(3H-TdR) incorporation, electronic microscopy, immunohistochemistry and in situ hybridization assays were used. The results showed that after DDPH administration (50 mg i.g. q.d. x8 wk to SHR), morphological change of VSMC was antagonized, blood pressure and 3H-TdR incorporation were significantly reduced. Furthermore, DDPH was shown to reverse the enhanced antigen PDGF-B and bFGF and reverse the reduced expression of oncogene c-sis and c-myc in SHR. These results suggest that DDPH may inhibit VSMC proliferation in SHR, which may be relevant to the molecular biological mechanism of growth factors and oncogene control.