Tanshinone IIA Ameliorates Trinitrobenzene Sulfonic Acid (TNBS)-Induced Murine Colitis |
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Authors: | Aiping Bai Nonghua Lu Yuan Guo Xianmin Fan |
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Institution: | (1) Department of Gastroenterology, The First Affiliated Hospital, Nanchang University, Nanchang City, 330006, China;(2) Department of Pharmacy, The First Affiliated Hospital, Nanchang University, Nanchang City, 330006, China;(3) Department of Medicine, The Affiliated Hospital, Luzhou Medical College, Luzhou City, 646000, China |
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Abstract: | Inflammatory bowel diseases are characterized by proinflammatory cytokines, oxidative stress, and tissue damage. Recently,
tanshinone had been shown to act as an antioxidant, and to have anti-inflammatory bioactivity. The study was carried out to
investigate the effect of tanshinone IIA on the inflammatory response of experimental colitis. Murine colitis was induced
by trinitrobenzene sulfonic acid (TNBS). Ten or 20 mg tanshinone IIA was administrated to mice 4 h before the induction of
colitis, and repeated daily until the mice were sacrificed. Colonic inflammation was examined by histological analysis, myeloperoxidase
(MPO) activity, and the production of proinflammatory cytokines in colonic tissue. Activation of nuclear factor-kappa B was
identified by western blot and immunohistochemistry, and oxidative stress was shown by glutathione (GSH) level in tissue.
The mice with colitis treated by tanshinone IIA showed less tissue damage, lower MPO activity, less production of TNF-α and
IL-1β, a higher level of GSH in colonic tissue, and downregulated activation of nuclear factor-kappa B in lamina propria mononuclear
cells, compared with those of the untreated colitis group. Our data indicates that tanshinone IIA inhibits inflammatory response
of colitis by downregulating the production of proinflammatory cytokines, and attenuating oxidative stress, which suggests
that tanshinone IIA may be a new potential management for inflammatory bowel diseases. |
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Keywords: | Tanshinone IIA Experimental colitis Nuclear factor-kappa B Proinflammatory cytokine Oxidative stress |
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