新清开灵注射液对大鼠脑出血后脑水肿的影响

目的 观察新清开灵注射液对胶原酶诱导大鼠脑出血后脑水肿及相关环节的影响。方法 用Ⅶ型胶原酶诱导大鼠脑出血，造模成功后腹腔注射新清开灵注射液，48h后用干湿重法检测脑含水量，用原子分光光度法检测脑组织中Ca元素含量，用ELISA检测血清中基质蛋白酶-9（MMP-9）的含量，用液相竞争放免法检测脑组织匀浆中肿瘤坏死因子-α（TNF-α）的含量。结果 新清开灵注射液能明显降低胶原酶诱导大鼠脑出血后脑含水量、Ca元素、血清MMP-9和脑TNF-α的含量。结论 新清开灵注射液阻断Ca^2＋超载、保护基底膜、消除炎症等各个方面来同时减轻细胞毒性脑水肿和血管源性脑水肿。

Effect of New Qingkailing Injection on Cerebral Edema Follow ing Intracerebral He morrhage in Rats

OBJECTIVE: To investigate the effect of New Qingkailing injection (NQKLI) on cerebral edema following intracerebral hemorrhage (ICH) induced by collagenase VII in rats. METHODS: After being established into ICH model by collagenase VII, rats were treated with NQKLI by intraperitoneal injection. Forty-eight hrs later, brain contents of water was detected with wet and dry method, calcium content in brain was detected by atomic spectrophotometer, tumor necrosis factor-alpha (TNF-alpha) in brain tissue was determined by liquid competitive ibhibitory immunoassay, and serum matrix metalloproteinase-9 (MMP-9) were detected with enzyme-linked immunosorbent assay (ELISA) respectively. RESULTS: NQKLI reduced the contents of water, calcium and TNF-alpha content of brain tissue and serum MMP-9 in rats with ICH. CONCLUSION: NQKLI could alleviate both vasogenic and cytotoxic cerebral edema by prohibiting calcium over-load, protecting basilar membrane and eliminating inflammation.