半夏厚朴汤醇提物对大鼠慢性抑郁模型的影响

目的 :研究半夏厚朴汤醇提物对大鼠慢性抑郁模型 (CMS)的影响 ,探索其抗抑郁机制。方法 :以 1%蔗糖水摄入量作为指标 ,慢性给予各种低强度复合刺激 ,造成大鼠慢性抑郁模型。按试剂盒酶法测定血脂 ;采用乳酸脱氢酶 (LDH)释放法测定脾细胞内自然杀伤细胞活性 ;采用邻苯三酚自氧化法测定红细胞内超氧化物岐化酶活性 ;按试剂盒显色法测定血清和组织中一氧化氮合酶活性 ;采用硫代巴比妥酸法测定心脏中丙二醛含量。结果 :在大鼠CMS模型中半夏厚朴汤醇提物可增加动物蔗糖摄入量 ;增加其脾脏自然杀伤细胞活性 ;升高血清中高密度脂蛋白 (HDL C)水平 (P<0 .0 5 ) ,降低甘油三酯水平 (P<0 .0 0 1) ;降低血红细胞内超氧化物岐化酶活性及血清和肝组织中一氧化氮合酶活性 ;同时抑制组织中脂质过氧化程度 ,降低心肌组织中丙二醛含量。结论 :半夏厚朴汤醇提物通过多途径而达到抗抑郁作用。

The Effects of Banxia Houpu Decoctionin a Chronic Mild Stress Model of Depression

Objective: To study the effect of Banxia Houpu Decoction in a chronic mild stress model of depression and investigate the antidepressive mechanism. Method: With consumption of a 1% sucrose solution as an index, and by subjecting rats to a variety of mild stressors for a prolonged period of time, a chronic mild stress model was developed. The levels of the blood lipid were measured by blood lipid kits, the natural kill (NK) cell activity in the spleen was measured with the method of the enzyme lactate dehydrogenase (LDH) released, the superoxide dismutase (SOD) activity in red blood cell was assayed by the Autoxidation of Pyrogallol method, the NO synthase (NOS) activity in serum and tissue was measured by NOS kits, and the content of malondialdedyde(MDA) was measured by MDA kits. Result: Banxia Houpu Decoction significantly increased the consumption of sucrose solution, increased the level of the high density lipoprotein (HDL-C), decreased the level of the Triglyceride(TG) in serum, enhanced the activity of the NK in spleen, decreased the activity of the SOD in red blood and the activity of the NOS in serum and tissue, and reduced the content of MDA in tissue by effect on lipid Peroxidation in CNS model of depression. Conclusion: Banxia Houpu Decoction has antidepressant effect in different ways.