抗纤舒肺颗粒对博来霉素诱导大鼠肺纤维化的影响

目的探讨抗纤舒肺颗粒对博来霉素所致大鼠肺间质纤维化的治疗作用及其机制。方法40只大鼠随机分为空白组、模型组、激素组和抗纤舒肺颗粒组,以气管内注入博莱霉素制作动物模型,于当天开始每天给药,分别于第5,25天处死5只,取肺组织进行免疫病理学检查及血浆黏度、全血黏度、胶原蛋白沉积检测。结果肿瘤坏死因子-a(TNF-α)在肺组织中的表达激素组和抗纤舒肺颗粒组均弱于模型组;第5天激素组和抗纤舒肺颗粒组无差异,但第25天有显著性差异。全血黏度、血浆黏度、纤维蛋白原模型组高于空白组;中药组第25天低于激素组,部分指标低于模型组;激素组高于模型组。胶原蛋白沉积的含量抗纤舒肺颗粒组及激素组明显低于模型组,其中抗纤舒肺颗粒组较激素组第25天下降更明显。结论抗纤舒肺颗粒通过抑制血小板活化、胶原蛋白合成及TNF-α表达,达到抗肺间质纤维化的作用;同时可改善肺间质纤维化形成中的高凝状态而改善肺内微循环,避免激素使用时引起的血液黏度增高,具有明显的抗肺间质纤维化作用。

Effect of Kangxianshufei granule on pulmonary interstitial fibrosis rat model induced by bleomycin

Objective It is to approach the therapeutic effect and mechanism of Kangxianshufei granule on the pulmonary interstitial fibrosis rat model induced by bleomycin.Methods 40 rats were randomly divided into 4 groups: blank control group,model group,glucocorticoid group and Kangxianshufei granule group.Medicine was given daily since models were established by injecting bleomycin into rats' trachea;5 rats were killed on the 5th day and 25th day respectively.The Immune and histopathologic examination of lung tissue were made,and the plasma viscosity, whole blood viscosity and collagen deposition?were detected.Results The expression of tumor necrosis factor-α(TNF-α) in lung tissue of glucocorticoid group and Kangxianshufei granule group were both smaller than that of model group;there were no significant difference between glucocorticoid group and Kangxianshufei granule group on the 5th day,but were significant difference on the 25th day.The levels of plasma viscosity,whole blood viscosity and collagen deposition?of model group were greater than that of blank control group;and the levels of Kangxianshufei granule group were lower than that of glucocorticoid group,and it's some indicator were lower than that of model group in the 25th day;and the levels of glucocorticoid group were higher than that of model group.The content of collagen deposition of Kangxianshufei granule group and glucocorticoid group were lower than that of model group obviously,and the content of Kangxianshufei granule group was lower more clearly on the 25th day.Conclusion Kangxianshufei granule has the function of anti pulmonary interstitial fibrosis by inhibiting platelet activation,collagen protein synthesis and TNF-α expressing;at the same time,it can improve the microcirculation in lung by improving the hypercoagulable state?in the pulmonary interstitial fibrosis progress,avoid the blood viscosity increasing caused by using of glucocorticoid,has apparent function anti pulmonary interstitial fibrosis.