恒稳电磁场对体外培养的日本血吸虫童虫细胞LDH的影响

目的以乳酸脱氢酶(lactate dehydrogenase,LDH)为评价指标,探讨恒稳电磁场对感染21d虫龄的日本血吸虫童虫培养细胞LDH的影响,筛选恒稳电磁场作用的最适强度与时间。方法将虫龄为21d的日本血吸虫童虫细胞接种于小盖玻片上,培养于常规培养基中。接种后第2d,将一部分细胞分别置于强度为0Gs(对照)、5Gs、10Gs、15Gs、20Gs、25Gs的恒稳电磁场中作用48h;另一部分置于20Gs磁场强度中分别作用为0h(对照)、12h、24h、36h、48h、60h、72h。然后运用酶细胞化学方法对日本血吸虫童虫培养细胞进行LDH染色,Olympus-BH2显微镜下观察并拍照,HIPAS-2000图像分析仪测定其含量,并作统计分析。结果日本血吸虫童虫培养细胞经不同强度恒稳磁场处理后其LDH着色均比对照组深,其中20Gs组的着色最深,其次是15Gs组,再是10Gs组,5Gs组与25Gs组细胞的着色相似,为最浅;统计分析显示,恒稳磁场处理后的各组培养细胞其LDH活性与对照组之间的差异均显著(q5/0=32.7054,q10/0=51.1946,q15/0=74.6917,q20/0=82.5062,q25/0=33.5342,P<0.01);各处理组之间两两比较,除5Gs与25Gs组间差别不显著(q5/25=0.4181,P>0.05)外,其余各组之间差异均有统计学意义(q5/20=49.5414,q5/15=41.4658,q5/10=18.33163,q10/25=18.1466,q10/20=31.3085,q10/15=23.0460,q15/25=41.5878,q15/20=8.5468,q20/25=49.7640,P<0.01)。相同磁场强度作用不同时间后,随着作用时间的延长,细胞的LDH逐渐变深,48h时达到最深,之后逐渐变浅;统计分析表明,不管磁场作用多长时间,培养细胞的LDH活性均明显强于对照组(P<0.01);各实验组之间两两比较亦然。结论恒稳电磁场可以明显增强日本血吸虫童虫细胞的LDH活性,其最佳作用强度与时间分别为20Gs、48h。

Selection of optimal condition of static electromagnetic field to the cultured cells from Schistosoma japonicum

To select the optimal condition of static electromagnetic field to lactate dehydrogenase(LDH) of the cultured cells from Schistosoma japonicum(S.japonicum). The cells from S.japonicum were inoculated on the small glass cover slips cultured in routine media. The cultured cells were randomly divided into test and control groups after they were cultured for 2 days. At first, the cells in the test group were cultured similarly to the ones in control group except that they were treated in 5Gs~20Gs static electromagnetic field for 48 hours; then, test group were cultured for 12~72hours in the optimal intensity. The optimal condition of static electromagnetic field is 20Gs and for 48 hours. (P<0.01, except q5/25= 0.4181,P>0.05), and (P<0.01). Static electromagnetic field can accelerating S.japonicum cellular metabolizability, respectively.