丹参多酚酸盐对健康人血小板内皮型一氧化氮合酶活性的影响

目的观察不同浓度的丹参多酚酸盐(depside salt from Salvia Miltiorrhiza)对正常人血小板内皮型一氧化氮合酶(eNOS)活性的影响。方法采集健康人外周静脉血,用凝胶色谱柱法纯化血小板,同位素二步色谱法测定血小板eNOS的活性。将收集的血小板分别与eNOS激动剂组织胺和(或)eNOS抑制剂N-ω-硝基左旋精氨酸甲酯盐酸盐(L-NAME)或不同浓度丹参多酚酸盐一起孵育30min后测定血小板eNOS活性。结果组织胺可以明显增加eNOS活性;L-NAME可以明显抑制基础状态下血小板eNOS活性以及组织胺刺激后eNOS活性的增加;丹参多酚酸盐在浓度0.1～10mg/L间呈浓度依赖性增加血小板eNOS活性(P<0·05);浓度达10mg/L时这种刺激作用达高峰,更高浓度时血小板eNOS活性反而逐渐降低。结论丹参多酚酸盐在一定剂量范围(<10mg/L)内可以明显增加血小板eNOS活性。

Effects of Depside Salt from Salvia Miltiorrhiza on Platelet Endothelial Nitric Oxide Synthase Activity

Objective To observe the effect of depside salt from Salvia Miltiorrhiza on platelet endothelial nitric oxide synthase(eNOS) activity in health adults.Methods Peripheral venous blood was collected,platelets were isolated with gel-filtration chromatography and incubated with eNOS agonist histamine,eNOS inhibitor L-NAME and different concentrations of depside salt from Salvia Miltiorrhiza(0.1,1,10,100,1000 mg/L) for 30 minutes,then eNOS activity was measured as formation of 3H-L-citrulline from 3H-L-arginine.Results(1)Platelet eNOS activity was significantly inhibited after incubated platelets with L-NAME and increased after incubated with histamine(P<0.05).(2)Depside salt from Salvia Miltiorrhiza in the range of 0.1 to 10 mg/L significantly stimulated platelet eNOS activity in normal subjects(P<0.05),however,with concentration higher than 10 mg/L,it switch to inhibit platelet eNOS activity.Conclusion Platelet eNOS activity was evidently increased by incubating with the low-concentration(<10 mg/L) of depside salt from Salvia Miltiorrhiza.