氯化汞对金黄地鼠胚胎发育的影响

目的 探讨氯化汞(HgCl2)对金黄地鼠胚胎发育的影响.方法 实验组于妊娠第6天开始经口灌服不同浓度的氯化汞水溶液,连续灌服5d,对照组在同一时间经口灌服蒸馏水.于孕第14天处死取胎,计录活胎数、死胎数、吸收胎数、植入数、畸形数,对活胎逐个检查外观并称重,测量其顶臀长;用HE染色方法对第8,9,10天胎鼠神经管进行形态学观察.结果 与对照组比较,经口灌服2mg/kg体重的氯化汞水溶液,对金黄地鼠胚胎体重和顶臀长的影响无显著差异,3mg/kg体重和4mg/kg体重的氯化汞水溶液对金黄地鼠胚胎的发育有一定的抑制作用,随着氯化汞剂量的增加,体重相应降低,但各实验组均未发现胎鼠畸形,HE染色结果显示,神经管形态与对照组相比无显著差异.结论 氯化汞对金黄地鼠胚胎发育有一定抑制作用,未检测到各种畸形.

The Influence of Mercuric Chloride on Embryonic Development of Golden Hamster

Objective To explore the influence of mercuric chloride on embryonic development of golden hamster.Methods Hamsters in the experimental group were intragastrically administrated daily with mercuric chloride for five days from the 6th day of gestation.Hamsters in the control group were intragastrically administrated daily with water at the same time.Golden hamster were sacrificed on the 14th day after pregnancy,then the fetuses were got.Living,dead,absorbed,implanted and defected fetuses in number were recorded.The living fetuses was checked,weighed.Their crown-rump length was measured.The morphological changes of neural tube of golden hamster fetuses were observed by hematoxylin-eosin staining.Results No evident change was observed in the 2mg/kg group,but embryonic development of golden hamster was inhibited in the 3mg/kg group and 4mg/kg group,their weight were decreased,but no defected fetuses were found in all the experimental group.There were no evident morphological changes of neural tube.Conclusion Mercuric chloride may inhibit embryonic development of golden hamster,but no defected fetuses were found.