| 荧光定量PCR与RT-PCR技术检测HCV-RNA的比较观察 |
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| 引用本文： | 林潮双,陈文思,卢建溪,谢俊强,柯伟民,高志良.荧光定量PCR与RT-PCR技术检测HCV-RNA的比较观察[J].中国实验诊断学,2000,4(4):176-178. |
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| 作者姓名： | 林潮双 陈文思 卢建溪 谢俊强 柯伟民 高志良 |
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| 作者单位： | 中山医科大学第三附属医院,传染科,广东,广州,510630 |
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| 基金项目： | 卫生部部属重点院校临床重点项目资助(课题编号:97040232,课题负责人:高志良.) |
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| 摘 要： | 目的 比较荧光定量ＰＣＲ与ＲＴ－ＰＣＲ（定性）检测不同检材中的ＨＣＶ－ＲＮＡ，评价荧光定量ＰＣＲ技术测定ＨＣＶ－ＲＮＡ水平的价值。方法 采用荧光定量ＰＣＲ及ＲＴ－ＰＣＲ（定性）技术同时检测了７１例血液透析患者的血清，血浆，淋巴细胞、尿液标本中ＨＣＶ－ＲＮＡ。结果 血清、淋巴细胞，尿液标本中ＨＣＶ－ＲＮＡ的荧光定量ＰＣＲ检出率（４６．３８％，８０．００％），分别高于相应标本中ＨＣＶ－ＲＮＡ的ＲＴ－Ｐ
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| 关 键 词： | 荧光定量PCR RT－PCR 丙型肝炎 HCV－RNA |
| 文章编号： | 1007-4287(2000)04-0176-03 |
| 修稿时间： | 1999年9月6日 |
Study on amplisensor quantitative PCR and RT-PCR in detecting HCV-RNA |
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| LIN Chao-shuang CHEN Wen-si,LU Jian-xi,et al..Study on amplisensor quantitative PCR and RT-PCR in detecting HCV-RNA[J].Chinese Journal of Laboratory Diagnosis,2000,4(4):176-178. |
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| Authors: | LIN Chao-shuang CHEN Wen-si LU Jian-xi |
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| Institution: | LIN Chao-shuang CHEN Wen-si,LU Jian-xi,et al. Department of Infectious Diseases,The Third Affilieded Hospital,Sun Yat-Sen University of Medical Sciences,Cuangzhou 510630 China |
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| Abstract: | Objective The aim of the study is to compare amplisensor quantitative PCR with RT-PCR (qualitative) in detecting HCV-RNA of different specimen and to evaluate the technique of amplisensor quantitative PCR. Methods Serum, urine and lymphocyte of 71 hemodialysis patients were tested for hepatitis C viral RNA by amplisensor quantitative PCR and RT-PCR (qualitative). Results Positive rate of HCV-RNA in seurm, lymphocyte, urine by amplisensor quantitative PCR (46.38%. 85.51%, 80.00%)was signdicanily higher than that by RT-PCR respectively (24.64%, 28.99%, 20.00%)(P<0.05).The rate of simultaneously positive HCV-RNA in serum and lymphocyte was significantly higher by amplisensor quantitative PCR (39.13%) than by RT-PCR (8.70%) (P<0.001). There was no relationship between the positive rate of HCV-RNA by RT- PCR and the quantilative level. Conclusion Compared with RT-PCR, amplisensor quantitative PCR technique has higher sen- sitivity in detecting HCV-RNA. |
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| Keywords: | Amplisensor quantitative PCR RT-PCR HCV-R |
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