维拉帕米对大鼠缺血—再灌流视网膜Glu—IR神经元的观察

探讨维拉帕米对大鼠缺血-再灌流视网膜Glu-IR神经元的作用。方法结扎双侧颈总动脉，3h后松结复流，分别于缺血前15min和再灌流前1min腹腔注射维拉帕米(5mg/kg/次)，对照组腹腔注射等量生理盐水。动物存活3d，4%多聚甲醛经心灌注，视网膜切片Glu免疫组织化学反应。结果正常大鼠视网膜内核层中有大量Glu-IR神经元，外核层、节细胞层部分细胞呈Glu-IR。缺血-再灌流损伤后，视网膜内核层中Glu-IR神经元较正常时显著减少，染色变淡，外核层、节细胞层未见Glu-IR(P＜0.05)。在缺血-再灌流期应用维拉帕米，视网膜中Glu-IR神经元明显增多，染色变深(P＜0.05)。结论视网膜Glu-IR神经元对缺血-再灌流损伤敏感，维拉帕米有助于视网膜Glu-IR神经元的存活及免疫活性的恢复。

INVESTIGATEION OFVERAPAMIL ON Glu-IR NEURONS OF TH E ISCHEMIA-REPERFUSION RETINA OF RAT

Objective:To investigation of verapamil on glutamate (Glu) immunoreactivity (IR) neurons of ischemia-reperfusion retina of rat.Methods:Both left and right carotid arteries were clamped for 3h.The carotid arteries of these animals were unclamped.Verapamil(5g/ml for verapamil-treated rats)or equal volume of the vehicle (for untreated animals) was peritoneal injected 15 minutes before 1 minute and after surger.After 3-day survival,the rats were sacrificed.ABC method of immunohistochemistry was used to study the changes of glutamate in retina.Results:Glu-IR neurons distributed the inner nuclear layer,outer nuclear layers and ganglion cell of retina in t he normal rats,After ischemia-reperfusion,Glu-IR neurons decreased and low stained in inner nuclear layer,no Glu-IR neurons in outer nuclear layer and ganglion cell (P<0.05);in verapamil treated animals,Glu-IR neurons increased and high stained (P<0.05).Conclusions:Glu-IR neurons in retina may belong to the sensitive to ischemia-reperfusion,administration of verapamil may benifit the survival and immunoreactivity of Glu-IR neurons in retina.