芪丑汤对肾大部切除大鼠白细胞黏附分子CD11b,CD44调控机制的研究

目的:探讨芪丑汤对肾大部切除大鼠白细胞黏附分子CD11b,CD44的调控机制。方法:以5/6肾大部切除为模型,将大鼠随机分成假手术组,肾大部切除组,包醛氧淀粉组,肾衰宁组,芪丑汤高、中、低剂量组。假手术组和肾大部分切除组每日给予等量生理盐水灌胃。包醛氧淀粉0.9 g.kg^-1;肾衰宁组0.37 g.kg^-1;芪丑汤高、中、低剂量组分别为53.1,26.6,13.3 g.kg^-1给药。采用流式细胞仪检测,用微量全血直接单标记荧光染色法观察各组大鼠白细胞黏附分子CD11b,CD44水平;用双抗体夹心ELISA法观察大鼠肿瘤坏死因子(TNF-α);同时观察尿素氮(BUN)、血肌酐(Scr)、左肾重、左肾重/体重的变化。结果:模型对照组大鼠CD11b,CD44,TNF-α表达增强,左肾重、左肾重/体重、BUN,Scr明显升高。芪丑汤治疗后,肾大部切除大鼠CD11b,CD44,TNF-α表达下降;BUN,Scr,左肾重、左肾重/体重明显降低。结论:芪丑汤对肾大部切除大鼠白细胞黏附分子CD11b,CD44,TNF-α水平有调控作用,能阻止白细胞浸润、炎症反应,抑制残余肾代偿性肥大,对肾大部切除大鼠的肾脏具有保护作用,且以芪丑汤中、低剂量组效果为好。

Regulation of leucocyte adhesion molecule CD11b,CD44 by Qichou decoction in the rats with largely partial nephrectomy

OBJECTIVE: To study on the regulation of leucocyte adhesion molecule CD11b, CD44 (CD11b, CD44) induced by Qichou decoction in the rats with kidney cut off largely. METHOD: The animal model was established by excising five sixth of kidney bilaterally. The rats were randomly divided into sham-operation group, model group with kidney cut off largely, the group treated by aldehyde oxygen amylum, the group treated with Shenshuaining and groups treated by high, moderate and low doses of Qichou decoction. The animals in the sham-operation group and model group were given with equated physiological brine. The dosages of aldehyde oxygen amylum and Shenshuaining were 0.9 g x kg(-1) and 0.37 g x kg(-1) respectively. The high, moderate and low dosages of Qichou decoction were 53.1, 26.6 g x kg(-1) and 13.3 g x kg(-1) respectively. The contents of leucocyte adhesion molecules including CD11b and CD44 in microserum were labeled by fluorescein and measured by flow Cytometry. Tumor necrosis factor (TNF-alpha)was detected by Dot-ELISA assay. RESULT: The expression of CD11b, CD44 and obviously in the model group as compared with sham-operation group. The weights of left kidney, the ratio of left kidney TNF-alpha was increased weight/body weight. BUN and Scr were also increased obviously. After the treatment with Qichou decoction, the contents of CD11b, CD44, TNF-a, BUN and Scr in the rats with kidney cut off largely were reduced significantly. Similarly, the weight of left kidney and the ratio of the left kidney weight/body weight were also decreased obviously. CONCLUSION: Leucocyte adhesion molecules including CD11b and CD44 in the rats with largely partial nephrectomy were significantly regulated by Qichou decoction. Qichou decoction can prevent immersion of the cells and inflammation, inhibit compensatory glomerular hypertrophy of the rest kidney, prevent the glomerular interstitial fibrosis, and protect the kidney in the rats with kidney cut off largely from further impairment.