PVP对人卵母细胞玻璃化冻融效果的影响

目的 探讨在玻璃化冷冻液中添加大分子物质PVP(polyvinyl pyrrolidone)对人卵母细胞冻融效果的影响.方法 玻璃化液中添加6% PVP者为A组,不添加者B组,未冻融者为对照组(C组),冻融后存活及对照组卵母细胞固定后进行免疫荧光染色,然后用Nikon CISI激光扫描共聚焦显微镜观察纺锤体、染色体形态...

the Effect of PVP Which was Added to Frozen Cryoprotective Agents in Vitrified Frozen-thawed Human Oocytes

【Abstract】Objective To explore the effect of macromolecule pvp to vitrified frozed-thawed human oocytes. Methods The study group A was added 6%PVP in the vitrified cryoprotective agents and the study group B wasn’t added any PVP. The fresh oocytes were as the control group（groupC） . After immunofluorescene staining , the study group survival oocytes and the control group oocytes were mounted on slides and observed under a Confocal Laser Scanning Microscope. Results The survival rate and the normal rates of spindle and chromosome configurations of group A were 83.3%（20/24）,10%（2/20）and 10%（2/20） . The survival rate and the normal rates of spindle and chromosome configurations of group B were 95.8%（23/24）,13%（3/23）and 13%（3/23）. There was no significant difference among the groups A,B and control group（P>0.05）.Conclusion PVP cann’t improve the effect of vitrified frozed-thawed human oocytes.