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Amifostine differentially modulates DNA damage evoked by idarubicin in normal and leukemic cells |
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| Authors: | B?asiak Janusz Gloc Ewa M?ynarski Wojciech Drzewoski Józef Skórski Tomasz |
| Institution: | a Department of Molecular Genetics, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland b Oncohaematology Unit, Institute of Pediatrics, Medical University of Lodz, Sporna 36/50, 91-738, Lodz, Poland c Department of Clinical Pharmacology, Medical University of Lodz, Rewolucji 1905r 37/39, 94-214, Lodz, Poland d College of Science and Technology, Temple University, 1900N 12th Street, Philadelphia, PA 19122, USA |
| Abstract: | Human lymphocytes, p53 protein-deficient acute promyelocytic leukemia cell line HL-60, murine pro-B lymphoid cell line BaF3 and its TEL/ABL-transformed clone cells were exposed to idarubicin with and without pre-treatment with amifostine. Idarubicin at 0.5–5 μM evoked DNA damage measured by the Comet assay. Amifostine at 14 mM decreased DNA-damaging effect of idarubicin in human lymphocytes and BaF3 cells, but increased the effect in TEL/ABL-transformed cells. Amifostine had no influence on the action of idarubicin in HL-60 cells. Our results suggest that the reaction of the cell to DNA damage may contribute to its diverse response to amifostine combined with anticancer drugs and that p53 and fusion tyrosine kinases may be involved in this diversity. |
| Keywords: | Amifostine Idarubicin DNA damage Comet assay TEL/ABL HL-60 |
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