单向肠灌流法研究芍药苷和刺芒柄花苷的肠吸收特性

目的:采用在体单向肠灌流法研究芍药苷和刺芒柄花苷在大鼠不同肠段的吸收特性,探索复方给药对单一活性成分吸收的影响.方法:采用高效液相色谱法进行含量测定和方法学验证.色谱柱:Shim Pack ODS C18 ( 250 mm × 4.6 mm,5 μm );流动相:测定芍药苷时为甲醇-乙腈-1.0 mL·L-1磷酸水溶液...

Study on intestinal absorption characteristics of paeoniflorin and ononin by in situ single-pass intestinal perfusion

Objective:To study the absorption characteristics of paeoniflorin and ononin in different intestinal segments of rats and explore the effect of compound administration on the absorption of single active ingredient by in situ single-pass intestinal perfusion.Methods:An HPLC system was utilized to determine the contents of paeoniflorin and ononin.Shim Pack ODS C18 column(250 mm×4.6 mm,5μm)was adopted;the mobile phases were methanol-acetonitrile-1.0 mL·L^-1 phosphoric acid aqueous solution(15∶15∶70)for paeoniflorin,and acetonitrile-1.0 mL·L^-1 formic acid aqueous solution(55∶45)for ononin,respectively;the flow rate was 1.0 mL·min^-1;the detection wavelength was 230 nm for paeoniflorin and 254 nm for ononin,respectively;the column temperature was 30℃.The main absorption pathway was investigated by comparing the effective permeability coefficient(Peff)values from the same drug concentration in different intestinal segments,and the different drug concentration in the same intestinal segment.Verapamil was added in the perfusion to study the influence of glycoprotein inhibitor on duodenal Peff.The influence of difference administration form on the absorption of single active ingredient was compared with compound form perfusion.Results:The linear ranges of paeoniflorin and ononin were both 0.5-60μg·mL^-1,and the peak area showed a good linear relationship with the concentration of corresponding compounds,r1=0.9999 for paeoniflorin and r2=0.9998 for ononin;intra-day precision RSDs were less than 2.5%and 2.9%,inter-day precision RSDs were all less than 3.1%,stability RSDs was less than 3.6%and 3.3%,respectively.The order of Peff value in rat small intestine from large to small was duodenal,jejunum and ileum.Verapamil had no significant influence on the Peff of paeoniflorin and ononin in the duodenum.There were significant differences in Peff of different concentrations of perfusion fluid,with the phenomenon of concentration saturation.Peff of paeoniflorin and ononin with compound perfusion(Buyang Huanwu decoction)were higher than those of single component perfusion.Conclusion:Duodenum is the best intestinal segment for the absorption of paeoniflorin and ononin.Compound administration can improve the absorption of some effective components in the intestinal tract.