甘姜苓术汤HPLC指纹图谱及含量测定方法的建立

目的:制备甘姜苓术汤对应实物,建立适用于甘姜苓术汤对应实物HPLC含量测定以及指纹图谱方法.方法:依照古籍记载制备甘姜苓术汤对应实物;应用HPLC对15批对应实物进行有效成分含量测定和指纹图谱建立,流动相为乙腈-0.05％磷酸水,梯度洗脱,检测波长237 nm.结果:建立甘姜苓术汤的HPLC含量测定和指纹图谱的分析方法...

Establishment of HPLC fingerprint and content determination method of Ganjianglingzhu decoction

Objective:To prepare the corresponding physical products of Ganjiang Lingshu decoction,in order to establish a method for HPLC content determination and fingerprinting of the corresponding physical products of Ganjiang Lingzhu decoction.Methods:According to the ancient books,Ganjianglingzhu decoction was prepared,the contents of active components and fingerprint of 15 corresponding materials were established by HPLC,the mobile phase was eluted with acetonitrile-0.05%phosphate water gradient,and the detection wavelength was 237 nm.Results:The HPLC content determination and fingerprint analysis method of Ganjiang Lingshu decoction was established.The peak resolutions in the specificity test of glycyrrhizin,glycyrrhizic acid and 6-gingerol were all greater than 1.5;the RSDs of the precision test were 0.74%,0.65%,and 0.69%respectively;the RSDs of the repeatability test were 1.1%,0.86%,0.92%;the RSDs of the stability test were 0.43%,0.78%,and 3.0%;the recoveries were 99.7%,104.5%,and 95.1%,respectively.In the fingerprint of the corresponding physical product,glycyrrhizin was used as the reference peak,and 15 common peaks were determined.By comparison with the reference substance,it was identified that peak 4 was glycyrrhizin,peak 13 was glycyrrhizin,and peak 14 was 6-ginger.The similarity between the corresponding physical fingerprint and the control fingerprint was good,and both were greater than 0.9.The average content of glycyrrhizin,glycyrrhizic acid,and 6-gingerol in 15 batches of material standards was 24.5,35.6,and 1.6 mg·g^-1,respectively.Conclusion:The established fingerprint of Ganjianglingshu decoction and the content determination method for detecting the three components are simple,accurate and reliable,and can be used for quality control of its material standards.