TGF-β1 、TβR Ⅱ及cyclinD1 在大肠癌的表达及其意义

 目的　探讨TGF-β1 、TβR Ⅱ及cyclinD1 在大肠癌发生发展过程中的作用。方法　采用免疫组化SP 法从蛋白水平分析TGF-β1 、TβR Ⅱ、cyclinD1 在大肠腺瘤及大肠癌组织中的表达情况。结果　TGF-β1 蛋白阳性表达率在腺瘤组织为28. 6 % ,大肠癌组织中为57. 1 %( P < 0. 05) ; TβR Ⅱ在大肠癌组织中的表达率为51. 8 % ,低于腺瘤组织( P < 0. 01) ; TGF-β1 、TβR Ⅱ表达的改变与大肠癌浸润深度、淋巴结转移有关; TβR Ⅱ和cyclinD1 的表达呈负相关( P < 0. 05) 。结论　TGF-β1 的过表达与大肠癌侵袭转移演进密切相关; TβR Ⅱ的失表达不仅有助于提高细胞对TGF-β1 的生长抵抗,也增强了大肠癌的侵袭性;cyclinD1 在TGF-β1 的生长抵抗中可能发挥了某些作用。

Significance of TGF-β1, TβR Ⅱ and cyclinD1 Expression in Colorectal Carcinoma

Objective 　To investigate the role of TGF-β1 , TβR Ⅱ,cyclinD1 expression in colorectal carcinoma. Methods 　SP immunostaining technique was used to examine the expression of TGF-β1 , TβR Ⅱand cyclinD1 in colorectal adenomas and carcinomas. Results 　TGF-β1 was expressed positively in 28. 6 %colorectal adenoma tissues while 57. 1 % in carcinoma ( P < 0. 05 ) ; TβR Ⅱ was expressed positively 51. 8 % in colorectal carcinoma tissues which was lower than adenoma tissues ( P < 0. 01) ; The expressionof TGF-β1 、TβR Ⅱin colorectal tissues was associated with colorectal carcinoma infilt ration depth , lymph node metastasis ; A negative correlation was observed between the expression of TβR Ⅱ and that of cyclinD1 ( P < 0. 05) . Conclusion 　The overexpression of TGF2β1 was highly related to the invasion or metastasis of colorectal carcinoma ; Decreased expression of TβR Ⅱ was helpful not only to enhance cell growth resistance to TGF2β1 but increase the invasion of colorectal carcinoma ; cyclinD1 may played a role in the resistance of TGF-β1.