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| 高效液相色谱-电喷雾电离质谱法分析不同时期金银花中酸性成分及含量变化 | |
| 引用本文： | 刘永刚,卢建秋,高晓燕,乔延江,刘倩,孙严彤. 高效液相色谱-电喷雾电离质谱法分析不同时期金银花中酸性成分及含量变化[J]. 中国药房, 2006, 17(22): 1756-1757 |
| 作者姓名： | 刘永刚 卢建秋 高晓燕 乔延江 刘倩 孙严彤 |
| 作者单位： | 1. 吉林大学药学院,长春市,130021;北京中医药大学,北京市,100029 2. 北京中医药大学,北京市,100029 3. 吉林大学药学院,长春市,130021 |
| 摘 要： | 目的考察不同时期金银花中酸性成分的含量变化。方法采用高效液相色谱-电喷雾电离质谱法对金银花中酸性成分进行分析;高效液相色谱用梯度洗脱,质谱用负离子模式。结果不同时期绿原酸、异绿原酸及其同分异构体6种成分含量有一定变化。结论本方法简单、快速、重现性好,可为道地药材的评价研究提供依据。 |
| 关 键 词： | 高效液相色谱-电喷雾电离质谱法 金银花 异绿原酸 绿原酸 同分异构体 |
| 文章编号： | 1001-0408(2006)22-1756-02 |
| 收稿时间： | 2005-12-08 |
| 修稿时间： | 2006-02-15 |
Determination of Acid Ingredients and Content Variations in Flos Lonicerae at Different Time by HPLC- ESI - MSn |
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| LIU Yonggang,LU Jianqiu,GAO Xiaoyan,QIAO Yanjiang,LIU Qian,SUN Yantong. Determination of Acid Ingredients and Content Variations in Flos Lonicerae at Different Time by HPLC- ESI - MSn[J]. China Pharmacy, 2006, 17(22): 1756-1757 | |
| Authors: | LIU Yonggang LU Jianqiu GAO Xiaoyan QIAO Yanjiang LIU Qian SUN Yantong |
| Affiliation: | 1.College of Pharmacy, Jilin College, Changchun 130021, China; 2.Beijing University of TCM, Beijing 100029, China |
| Abstract: | OBJECTIVE:To study the content changes of the acid ingredients in Flos Ionicerae at different time.METHO_ DS:The acid ingredients in Flos Ionicerae were analyzed by HPLC-ESI-MSn.The HPLC was subjected to gradient elution and negtive ion mode was adopted in mass spectrum.RESULTS:The contents of 6 ingredients-chlorogenic acid,isochlorogenic acid and their isomers varied at different time.CONCLUSION:The method was proved to be simple,fast,reproducible,and it can provide bases for the evaluation of famous-region Flos lonicerae. |
| Keywords: | HPLC ESI MS^n Flos lonicerae Isochlorogenic acid Chlorogenic acid Isomers |
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