| 南瓜多糖的提取纯化及其分析 |
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| 引用本文： | 孔庆胜 蒋滢. 南瓜多糖的提取纯化及其分析[J]. 济宁医学院学报, 1999, 22(4): 37-39 |
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| 作者姓名： | 孔庆胜 蒋滢 |
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| 作者单位： | 济宁医学院生物化学教研室(孔庆胜)，苏州医学院(蒋滢) |
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| 摘 要： | 目的 寻求有效、安全的糖尿病食疗剂。方法 南瓜干粉经热水提取，Ｓｅｖａｇ 法弃蛋白，透析，乙醇沉淀，沉淀干燥物再经Ｓｅｐｈａｄｅｘ Ｇ－ １００ 柱层析纯化，即得南瓜多糖精品。用聚丙烯酰胺凝胶电泳及玻璃纤维纸电泳证明为均一体。结果 南瓜多糖精品的分子量为１－６×１０４ 。其用酸完全水解，经纸层析表明由Ｄ－ 葡萄糖、Ｄ－ 半乳糖、Ｌ－ 阿拉伯糖、木糖和Ｄ－ 葡萄糖醛酸组成。其摩尔比分别为０－１８１ ：０－０８３ ：０－０６９：０－０３１ ：０－１７５ 。结论 南瓜多糖可作为糖尿病食疗剂中的有效成分。
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| 关 键 词： | 南瓜多糖 提取 纯化 |
ISOLATION AND PURIFICATION OF POLYSACCHARIDE FROM THE PUMPKIN AND DETECTION OF ITS MONOSACCHARIDE |
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| Kong Qingsheng,Jiang Ying. ISOLATION AND PURIFICATION OF POLYSACCHARIDE FROM THE PUMPKIN AND DETECTION OF ITS MONOSACCHARIDE[J]. Jounal of Jinning Medical College, 1999, 22(4): 37-39 |
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| Authors: | Kong Qingsheng Jiang Ying |
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| Abstract: | Objective To find effective food therapeutics that imptoves diabetes mellitus.Methods From the Pumpkin,with hot water and purified by deproteinization and removal of lipid.Some chemical and physical properties of the polysaccheride were examined. It was homogeneous as judged by electrophoresis.Result The PP molecular weight was estimated to be 1.6 x 10 4 and acidic heteroglycan composed mainly of D-glucose,D-galactise,L-arabinose, Xylose and D-glucuronic aicd in the molar ratios of 0.181:0.083:0.069:0.031:0.175 respectively.Conclusion PP can be considered a potent compound that improves diabetes mellitus |
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| Keywords: | Pumpkins Polysaccharide Isolation Purification |
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