| 硫醇／金自组装膜上组装葡萄糖氧化酶及其应用 |
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| 引用本文： | 孙灏,王洪恩.硫醇／金自组装膜上组装葡萄糖氧化酶及其应用[J].济宁医学院学报,1999,22(1):4-7. |
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| 作者姓名： | 孙灏 王洪恩 |
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| 作者单位： | 东北师范大学生命科学学院(孙灏)，济宁医学院分子医药学实验室(王洪恩) |
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| 摘 要： | 目的研制一种新的电流式葡萄糖传感器。方法这种传感器的制作方法是固定葡萄糖氧化酶于聚合物复合膜中，并且这种复合膜形成于金电极上的硫醇自组装膜之上。葡萄糖氧化酶和锇—聚乙烯基吡啶配合物的复合膜是在硫醇自组装膜上混合葡萄糖氧化酶和锇－聚乙烯基吡啶形成。结果以上方法制成的多层有序膜中Ｏｓ配合物作为电子传递体，对葡萄糖的响应在１～２０ｍｍｏｌ／Ｌ范围内呈线性。结论这种酶电极具有高度的稳定性，并对抗坏血酸有较强的抗干扰能力。
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| 关 键 词： | 葡萄糖氧化酶 自组装膜 聚（4－乙烯基）吡啶锇配合物 |
IMMOBILIZED GLUCOSE OXIDASE AT THIOL/GOLD SELF-ASSEMBLED MONOLAYER AND ITS APPLICATIONS |
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| Sun Hao,Wang Hongen.IMMOBILIZED GLUCOSE OXIDASE AT THIOL/GOLD SELF-ASSEMBLED MONOLAYER AND ITS APPLICATIONS[J].Jounal of Jinning Medical College,1999,22(1):4-7. |
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| Authors: | Sun Hao Wang Hongen |
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| Abstract: | Objective A new amperometric enzyme electrode was prepared by immobilizing glucose oxidase in a polymer complex film based on thiol self-assembled monolayer at glod electrode Methods The film of glucose oxidase and Os-PVP(Osmium-polyvinylpyridine) complex were formed by mixing glucose oxidase and Os-PVP solution at thiol self-assembled monolayer Results Os complex acts as electron mediator, and its response to glucose was linear at a range of 1 to 20 mmol/L Conclusion This enzyme electrode shows low interference from ascorbic acid and exhibits higher stability. |
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| Keywords: | Self-assembled monolayer Glucose oxidase Osmic complex |
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