| 脐血源性T淋巴细胞在白血病小鼠体内存活及浸润状况的研究 |
| |
| 引用本文： | 王志效,黄海燕,耿微. 脐血源性T淋巴细胞在白血病小鼠体内存活及浸润状况的研究[J]. 中国医疗前沿, 2008, 3(24) |
| |
| 作者姓名： | 王志效 黄海燕 耿微 |
| |
| 作者单位： | 济宁市中心血站 |
| |
| 摘 要： | 目的研究脐血源性T淋巴细胞在小鼠体内的存活、浸润情况,为深入探讨脐血源性T淋巴细胞过继性输注治疗白血病提供实验和理论依据。方法24只红白血病小鼠随机分为对照组和试验组。将分离制备的脐血源性T淋巴细胞标记后经尾静脉移植入白血病小鼠体内,采用免疫荧光、免疫组织化学等方法观察脐血源性T淋巴细胞在小鼠体内的存活时间及浸润状况。结果①移植后第3、7、14天在实验组小鼠外周血、肝和脾组织中均可检测到人CD3+细胞和Brdu+细胞;②实验组小鼠肝、脾组织内均见CD25+细胞散在浸润。结论脐血源性T淋巴细胞能在白血病小鼠体内存活并趋化至白血病细胞浸润部位。
|
| 关 键 词： | 脐血源性T淋巴细胞 红白血病 BALB/C小鼠 过继性输注 免疫荧光染色 |
Study on the Survial and Infiltrated, Condition of Cord Blood-derived T Lymphocytes in Leukemic Mice |
| |
| WANG Zhi-xiao,HUANG Hai-yan,GENG Wei. Study on the Survial and Infiltrated, Condition of Cord Blood-derived T Lymphocytes in Leukemic Mice[J]. China Healthcare Innovation, 2008, 3(24) |
| |
| Authors: | WANG Zhi-xiao HUANG Hai-yan GENG Wei |
| |
| Affiliation: | WANG Zhi-xiao; HUANG Hai-yan; GENG Wei(The Blood Center of Jining; Jining; 272041; China); |
| |
| Abstract: | Objective To study the survival and infiltrated condition of cord blood derived-T lymphocytes(CBTLs)in mice,to provide the experimental and theoretical basis for study on treated leukemia by cord blood-derived T cells adoptive infusion.Methods 24 erythroleukemic mice were randomly divided into two groups,the control group(CG)and the experimental group(EG).Separated and prepared cord blood derived-T lymphocytes,and transplanted it into erythroleukemic mice via the tail vein injection after signed,in order to... |
| |
| Keywords: | Cord blood derived-T lymphocytes Erythroleukemia BALB/C mice Adopt infusion Immunofluorescent staining |
| 本文献已被 维普 万方数据 等数据库收录！ |
