| 用自制抗HRP－抗HBc双特异性单克隆抗体检测抗HBc的初步应用 |
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| 引用本文： | 田生和,王大坤,巫山,袁雪,陈善华. 用自制抗HRP－抗HBc双特异性单克隆抗体检测抗HBc的初步应用[J]. 现代免疫学, 1995, 0(4) |
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| 作者姓名： | 田生和 王大坤 巫山 袁雪 陈善华 |
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| 作者单位： | 卫生部武汉生物制品研究所 |
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| 摘 要： | 用杂交瘤融合法建立了抗ＨＲＰ－抗ＨＢｃ双特异性单克隆抗体（ＢｓＡｂ）杂交－杂交瘤细胞系，它分泌的腹水抗体经ＦＰＬＣ－离子交换层析梯度盐洗脱呈现三个主峰，抗体活性检测表明依次为抗ＨＲＰ、ＢｓＡｂ及抗ＨＢｃ。试用ＢｓＡｂ－ＨＲＰ复合物检测乙肝病例血样，与市售试剂盒初步比较，效果满意。
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| 关 键 词： | 亲本杂交瘤，BsAb，FPLC－离子交换层析，竞争ELISA |
The Elementary Application of Anti HRP-Anti HBc Bispecific Monoclonal Antibody in Detecting Anti-HBc |
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| Tian Shenghe,Wang Dakun,Wu Shan,Yuan Xue,Chen Shanhua. The Elementary Application of Anti HRP-Anti HBc Bispecific Monoclonal Antibody in Detecting Anti-HBc[J]. Current Immunology, 1995, 0(4) |
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| Authors: | Tian Shenghe Wang Dakun Wu Shan Yuan Xue Chen Shanhua |
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| Abstract: | A train secreting anti HRP-anti HBc bispecific monoclonal antibody(BsAb)was establishedby the fusion ofhybridomas.Its ascitic antibody was purified by FPLCion exchange chromatography employing a gradient saline elution which exhibited three major peaks and can be measured anti-HRP,BsAb,anti-HBc activity respectively.Blood samples detection using the BsAb-HRP combination comparing to a commercial detecting anti-HBc kit showed a satisfactory correspondence. |
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| Keywords: | parental hybridoma BsAb FPLC-ion exchange chromatography competitive ELISA |
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