人肝再生增强因子相互作用蛋白基因的克隆

目的：寻找与人肝再生增强因子(hALR)相互作用的蛋白质．探讨其在肝再生过程中的分子生物学机制。方法：采用酵母双杂交系统，以hALR作诱饵蛋白筛选预转化的人肝cDNA文库，对阳性克隆进行生物信息学分析。结果：筛选出6组与hALR具有特异性相互作用的蛋白基因，分别是：血清白蛋白、金属硫蛋白、Na／K-ATPase、硒蛋白P和两个未知功能基因的cDNA序列。结论：初步克隆了与hALR相互作用蛋白基因，为以后深入研究这些蛋白质与hALR之间的相互作用，进一步揭示hALR的作用机制奠定了基础。

The cloning of human augmentor of liver regeneration-interacting proteins

AIM: To screen human augmentor of liver regeneration(hALR)-interacting proteins and to explore the mechanisms of hALR in liver regeneration. METHODS: The open reading frame of hALR was used to construct the "bait" plasmid and the genes encoding hALR-interacting proteins were screened from the human liver cDNA library that was pretransformed into yeast Y187 by yeast two-hybrid system. Bioinformatic analysis of the sequences of the positive clones were performed. RESULTS: The positive clones encoding metallothionein, albumin, selenoprotein P, Na/K-ATPase, and 2 unknown proteins were screened out. CONCLUSION: The successful cloning of the genes encoding proteins interacting with hALR may pave the way for studying the interaction between the above proteins and hALR, and the molecular mechanisms of biological effect of hALR.