异基因外周血造血干细胞移植后造血细胞嵌合状态的分析

为评估异基因外周血造血干细胞移植（ALLO－PBSCT）后的植入状况，采用细胞遗传学分析结合短串联重复序列－聚合酶链反应（STR－PCR），对30例Allo-PBSCT病人进行了1－25个月的随访观察。结果表明，不同性别移植的23例，21例完全嵌合（CC），1例混合嵌合（MC），1例为完全受者核型；相同性别移植的慢性髓性白血病（CML）7例，除2例外，其余均未检测到Ph染色体；STR－PCR检测结果与染色体分析结果完全一致。说明两种方面具有互补性，可结合使用。

ANALYSIS OF HEMATOPOIETIC CHIMERISM STATE AFTER ALLOGENEIC PERIPHERAL BLOOD STEM CELL TRANSPLANTATION

To evaluate engraftment state after allogeneic peripheral blood stem cell transplantation (ALLO PBSCT), 30 patients who received ALLO PBSCT were studied using cytogenetic analysis and short tandem repeats polymerase chain reaction (STR PCR). Twenty one of the 23 patients who received sex mismatched donor marrows showed complete chimerism (CC), one showed mixed chimerism (MC), and one showed completely recipient karyotype. In the 7 patients of chronic myelogenous leukemia (CML) who received sex matched donor marrows, except 2 patients, the rest showed completely disappearance of Ph positive cells. The results from these two detection methods completely coincided mutually. STR PCR and cytogenetic analysis can be supplementary to each other and their results can be pooled together.