| 蜂毒素的纯化方法及体外抗肿瘤作用研究 |
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| 引用本文： | 刘岭,凌昌全,黄雪强. 蜂毒素的纯化方法及体外抗肿瘤作用研究[J]. 中国生化药物杂志, 2003, 24(4): 163-166 |
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| 作者姓名： | 刘岭 凌昌全 黄雪强 |
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| 作者单位： | 第二军医大学,长海医院,中医科,上海,200433 |
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| 基金项目： | 上海市科学技术委员会资助项目 (0 2DZ1910 9) |
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| 摘 要： | 目的从蜜蜂毒中分离纯化蜂毒素 ,观察其体外对多种肿瘤细胞的生长抑制作用。方法用SephadexG 2 5、SephadexG 5 0、SephadexG 75进行 3步色谱法从蜜蜂毒中分离纯化蜂毒素 ,以高效液相色谱法进行含量测定 ;溴化四唑蓝比色法观察蜂毒素对SMMC 772 1、BEL 74 0 2和Hep 3B等 3种肿瘤细胞的生长抑制作用。结果高效液相色谱测定结果显示供试品为单峰 ,保留时间与对照品一致 ;蜂毒素剂量依赖性地抑制 3种受试肿瘤细胞的生长。结论以凝胶过滤色谱可制得高纯度的蜂毒素 ,蜂毒素在体外呈现出明显的抗肿瘤作用
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| 关 键 词： | 蜂毒素 凝胶过滤色谱 高效液相色谱 抗肿瘤活性 |
| 文章编号： | 1005-1678(2003)04-0163-04 |
| 修稿时间： | 2002-09-13 |
Study on purification of melittin and its effect on anti-tumor in vitro |
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| LIU Ling,LING Chang-quan,HUANG Xue-qiang. Study on purification of melittin and its effect on anti-tumor in vitro[J]. Chinese Journal of Biochemical Pharmaceutics, 2003, 24(4): 163-166 |
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| Authors: | LIU Ling LING Chang-quan HUANG Xue-qiang |
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| Abstract: | PurposeTo isolate and purify melittin from bee venom and to investigate its anti-tumor effects in vitro.MethodsMelittin was isolated and purified from bee venom by gel filtration chromatography on Sephadex G-25,Sephadex G-50 and Sephadex G-75, and the purity was tested by HPLC. The cytotoxicity of Melittin against established tumor cell lines,SMMC-7721,BEL-7402 and Hep-3B cell, was determined using the MTT assays.ResultsThe target sample was found to be consistent with melittin in retention time on HPLC. Melittin showed an anti-tumor effect in a dose-dependent patten for tumor cells in vitro.ConclusionMilittin prepared by a combination of gel filtration chromatography had high purity. Melittin has remarkable anti-tumor activity in vitro against established tumor cell lines. |
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| Keywords: | melittin gel filtration chromatography HPLC anti-tumor activity |
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