离子色谱法同时测定葡萄糖酸钙及其杂质(Cl-,SO42-)的含量

本文采用Waters ILC-1离子液相色谱仪,IC-PAK A50×4.6mm色谱柱,pH6.61×10^-3mol/L邻苯二甲酸溶液为流动相,430型电导检测器检测,由外标法和外标单点校正法同时测定了葡萄糖酸钙及其杂质(Cl^-,SO₄^2-)的含量。方法简便、快速,测定结果与中国药典法一致。外标法和外标单点校正法测定Cl^-,SO₄^2-的平均回收率为100.8%和100.2%,相关系数分别为0.9951(n=8)和0.9961(n=8)。

SIMULTANEOUS QUANTITATIVE DETERMINATION OF CALCIUM GLUCONATE AND ITS IMPURITIES (CI- SO42-) BY ION CHROMATOGRAPHY

Ion chromatographic separation and determination of calcium gluconate and its impurities (Cl^-, SO₄^2-) were studied. Waters ILC-1 Ion/Liquid chromatograph, 50 × 4.6 mm ID stainless steel IC-PAK A column were used, pH 6.6 1 × 10^-3 mol/L phthalic acid as mobile phase at a flow rate of 1.2 ml/min, 430 conductivity detector with 5μS FS. The results obtained by external standard method, external standard single-point calibration method and Chinese Pharmacopoeia method were in good agreement. The average recoveries of the two proposed methods were 100.8% (C1^-) and 100.2% (SO₄^2-), the correlation coefficients were 0.9951 (C1^-) and 0.9961 (SO₄^2-) (n=8) respectively. This method is simple, relatively fast and can be used for simultaneous quantitative determination of this drug and its impurities.