12种实验室保存菌种对常用消毒剂的抗性分析

目的:了解本实验室所存菌种对常用消毒剂的抗性,从而合理使用消毒剂,避免实验室感染。方法:采用悬液定量杀菌试验分别测定75%乙醇、3%过氧化氢、碘伏和533消毒液对12种实验室保存菌种的平均杀灭对数值,采用K-B纸片法测定碘伏和533消毒液对12种菌种及其标准株的最低抑菌浓度(MICs)。结果:碘伏作用1 min时对12种菌种的杀灭对数值均>5.00;而533消毒液需作用8 min、75%乙醇和3%过氧化氢需作用40⁵0 min才能达到同样效果。碘伏和533消毒液对12种菌种的MICs分别为37.250 min才能达到同样效果。碘伏和533消毒液对12种菌种的MICs分别为37.2¹73.6、548173.6、548¹ 307 mg/L,其中533消毒液对12种菌种均表现出比标准株更高的MICs。结论:12种菌种对碘伏均未产生抗性,部分菌种对75%乙醇、3%过氧化氢和533消毒液产生抗性,说明应根据不同的消毒对象选择不同的消毒剂,且配制准确的浓度,才能达到良好的消毒效果。

Analysis of Resistance to Common Disinfectants of 12 Kinds of Laboratory Stored Strains

OBJECTIVE：To detect the resistance to common disinfectants of 12 kinds of laboratory stored strains in our laboratory so as to use disinfectants rationally and avoid laboratory infection.METHODS：Suspension quantitative germicidal test was used to determine average germicidal logarithmic value of 75% ethanol,3% H2O2,iodophor and＂533＂disinfectants to 12 kinds of laboratory stored strains.K-B method was applied to test the minimal inhibitory concentrations（MICs）of iodophor and 533 disinfectants to 12 kinds of laboratory stored strains and standard strains.RESULTS：The average germicidal logarithm values of iodophor in 1 min were above 5.00,so did by＂533＂disinfectants in 8 min,3% H2O2and 75% alcohol in 40-50 min.MICs of iodophor and＂533＂disinfectant to 12 kinds of strains were 37.2-173.6 mg/L and 548-1 307 mg/L,respectively.＂533＂disinfectants have higher MICs value to 12 kinds of strains,compare with standard strains.CONCLUSIONS：12 kinds of strains have no resistant to iodophor,while some strains are resistant to 75% ethanol,3% H2O2and＂533＂disinfectants.The disinfectants should be selected according to different objects,and sound disinfection effect can be achieved by accurate concentration.