丹参酮ⅡA对TNF-α诱导的ECV304细胞NF-κB、IκB-α表达及粘附分子ICAM-1、VCAM-1mRNA表达的影响

目的探讨丹参酮ⅡA对血管内皮细胞株ECV304NF-κB、IκB-α及粘附分子ICAM-1、VCAM-1mRNA表达的影响,以阐明其抗动脉粥样硬化作用及机制。方法通过建立TNF-α诱导的ECV-304细胞损伤模型,以抗氧化剂吡咯烷二硫代氨基甲酸盐(PDTC)做为对照,间接细胞ELISA方法定量检测NF-κB、IκB-α的表达,RT-PCR方法检测各组细胞ICAM-1、VCAM-1mRNA的表达。结果TNF-α可增加ECV304细胞转录因子NF-κB的表达,同时降低其抑制因子IκB-α的表达,低浓度的丹参酮ⅡA对TNF-α引起的ECV304细胞NF-κB的表达升高无明显抑制作用,但可增加其IκB-α的表达;高浓度的丹参酮ⅡA可明显抑制NF-κB的表达,同时增加其抑制因子IκB-α的表达。同时丹参酮ⅡA抑制TNF-α诱导的ECV304细胞ICAM-1、VCAM-1mRNA表达。结论丹参酮ⅡA可通过抑制转录因子NF-κB的激活及其相关粘附分子ICAM-1、VCAM-1mRNA表达,这有利于抑制动脉粥样硬化过程中的炎症从而发挥抗动脉粥样硬化作用。

The effect of Tanshinone IIA on the expression of NF-κB、IκB-α and the mRNA of ICAM-1 and VCAM-1 of ECV304 induced by TNF-α

Aim To illustrate the role of Tanshinone IIA(TanIIA)extracted from dan-shen root in atherosclerosis and its different targets by investigating the effect on the expression of NF-κB、IκB-α and the mRNA of ICAM-1 and VCAM-1 in human umbilical vascular endothelial cell 304(ECV304).Mehods The TNF-α-induced cultured ECV-304 cell injury model was established to analyze the expression of NF-κB、IκB-α by cell ELISA method and the mRNA of ICAM-1 and VCAM-1 by RT-PCR method of the cells cultured with different denses of TanIIA.Results The results of ELISA method showed that the expression of the NF-κB was reduced significantly and the expression of the IκB-α was enhanced significantly in ECV304 which were cultured with higher denses of TanIIA.The results of RT-PCR method showed that the mRNA of ICAM-1 and VCAM-1 induced by TNF-α was significantly refrained.Conclusions TanIIA has a significant effect on inhibiting the mRNA of ICAM-1 and VCAM-1 induced by the NF-κB activation,so it can play an impotant role in resisting atherosclerosis.