Human Antigen Leucocyte (HLA)-G and HLA-E are differentially expressed in pancreatic disorders |
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| Authors: | Bruna Cristina Bertol Fabrício César Dias Deisy Mara da Silva Leandra Náira Zambelli Ramalho Eduardo Antônio Donadi |
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| Affiliation: | 1. Postgraduate Program of Basic and Applied Immunology, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, ZIP Code: 14049-900 Ribeirão Preto, Brazil;2. Department of Medicine, Division of Clinical Immunology, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, ZIP Code: 14049-900 Ribeirão Preto, Brazil;3. Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, ZIP Code: 14049-900 Ribeirão Preto, Brazil |
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| Abstract: | BackgroundLittle information is available regarding the expression of the immunomodulatory Human Leukocyte Antigen (HLA)-G and -E molecules in pancreatic disorders.AimTo analyze HLA-G and -E expression in specimens of alcoholic chronic pancreatitis (ACP), idiopathic chronic pancreatitis (ICP), type 1 (T1D) and type 2 diabetes (T2D) and in histologically normal pancreas (HNP).MethodsHLA-G and -E expression (ACP = 30, ICP = 10, T1D = 10, T2D = 30 and HNP = 20) was evaluated by immunohistochemistry in three different areas (acini, islets and inflammatory infiltrate).ResultsAcini and islets from HNP specimens exhibited higher HLA-G and -E expression compared to corresponding areas from all other patient groups. In inflammatory infiltrate, HLA-G and -E expression was observed only among the pancreatic disorders. We observed higher HLA-G and -E expression in acini from T2D compared to ACP, as well as higher HLA-G expression compared to ICP.ConclusionThe decreased expression of HLA-G and -E in islets and acini together with the expression of these molecules in the inflammatory infiltrating cells were shared features among chronic inflammatory and autoimmune pancreatic disorders evaluated in this study, possibly reflecting tissue damage. |
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| Keywords: | Corresponding author at: Ribeirão Preto Medical School, University of São Paulo, 3900 Bandeirantes Avenue, ZIP Code: 14049-900 Ribeirão Preto, SP, Brazil. Chronic pancreatitis Major histocompatibility complex Immune checkpoint molecules HLA Human Antigen Leucocyte CP Chronic pancreatitis ACP Alcoholic chronic pancreatitis ICP Idiopathic chronic pancreatitis T1D Type 1 diabetes T2D Type 2 diabetes HNP Histologically normal pancreas IHC Immunohistochemistry NK Natural killer H&E Hematoxylin and eosin PBS Phosphate-buffered saline mAbs Monoclonal antibodies 5′URR 5′ upstream region LCR Locus control region NF-κB Nuclear factor κB IFN Interferon LINE1 Long interspersed elements |
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