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LINC00205 promotes proliferation,migration and invasion of HCC cells by targeting miR-122-5p
Authors:Lei Zhang  Yun Wang  Jingjing Sun  Hongye Ma  Cheng Guo
Affiliation:1. Department of Critical Care Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;2. Department of Gastroenterology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China;3. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province 710061, China
Abstract:Long non-coding RNAs (lncRNAs) have been identified as crucial regulators in the tumorigenesis and progression of hepatocellular carcinoma (HCC). Recently, long intergenic non-protein coding RNA 205 (LINC00205) has been identified as a prognostic biomarker in HCC. However, the biological role of LINC0205 and its potential molecular mechanism are poorly investigated. Here, we found that the expression of LINC00205 was dramatically up-regulated in HCC tissues compared to adjacent nontumor tissues. Furthermore, the level of LINC00205 in both Hep3B and Huh7 cells was prominently higher than that in normal hepatic cell line LO2. Notably, the high expression of LINC00205 was strongly correlated with tumor size ≥5 cm, venous infiltration and advanced tumor stages. Functionally, LINC00205 knockdown obviously repressed the proliferation, migration and invasion of Hep3B and Huh7 cells in vitro. An inverse correlation between LINC00205 and miR-122-5p was detected in HCC tissues. Interestingly, LINC00205 knockdown increased the level of miR-122-5p in both Hep3B and Huh7 cells. Mechanistically, luciferase reporter assay demonstrated LINC00205 acted as a competing endogenous RNA (ceRNA) by directly interacting with miR-122-5p. More importantly, miR-122-5p overexpression significantly restrained the proliferation, migration and invasion of HCC cells. Collectively, our study provides solid evidence to support the oncogenic role of LINC00205 in HCC, which may be benefit for the improvement of HCC therapy.
Keywords:Corresponding author at: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, 277 Yanta West Road, Xi’an, Shaanxi Province 710061, China.  HCC  hepatocellular carcinoma  LncRNAs  long non-coding RNAs  MCM3AP-AS1  MCM3AP antisense RNA 1  FOXA1  forkhead box A1  CASC2  cancer susceptibility candidate 2  FBXW7  F-box and WD repeat domain containing 7  DSCR8  down syndrome critical region 8  ceRNA  competing endogenous RNA  FZD7  frizzled-7  LINC00205  long intergenic non-protein coding RNA 205  PDAC  pancreatic ductal adenocarcinoma  TCGA  The Cancer Genome Atlas  TNM  tumor-node-metastasis  MAT2A  methionine adenosyltransferase 2A  PTTG3P  pituitary tumor-transforming 3, pseudogene  CASC9  cancer susceptibility 9  HNRNPL  heterogeneous nuclear ribonucleoprotein L  LINC00205  HCC  miR-122-5p  Proliferation  Tumor metastasis
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