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Mast cell corticotropin-releasing factor subtype 2 suppresses mast cell degranulation and limits the severity of anaphylaxis and stress-induced intestinal permeability
Authors:Susan D&#x;Costa  Saravanan Ayyadurai  Amelia J Gibson  Emily Mackey  Mrigendra Rajput  Laura J Sommerville  Neco Wilson  Yihang Li  Eric Kubat  Ananth Kumar  Hariharan Subramanian  Aditi Bhargava  Adam J Moeser
Institution:1. Gastrointestinal Stress Biology Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, East Lansing, Mich;2. Department of Physiology, Michigan State University, East Lansing, Mich;3. Thurston Arthritis Research Center, University of North Carolina, Chapel Hill, NC;4. KeraFast, Boston, Mass;5. Comparative Biomedical Sciences Program, North Carolina State University, Raleigh, NC;6. Department of Pathology, Duke University, School of Medicine, Durham, NC;7. Department of Surgery and Osher Center for Integrative Medicine, University of California, San Francisco, Calif;8. Department of Surgery, East Bay, University of California, San Francisco, Calif
Abstract:
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  • Keywords:Corresponding author: Adam J  Moeser  DVM  PhD  Gastrointestinal Stress Biology Laboratory  Department of Large Animal Clinical Sciences  Neuroscience Program  Physiology  Michigan State University  East Lansing  MI 48824    Mast cell  corticotropin-releasing factor receptor  stress  anaphylaxis  allergy  intestinal permeability  BMMC  Bone marrow–derived mast cell  CRF  Corticotropin-releasing factor  Corticotropin-releasing factor subtype 1  Corticotropin-releasing factor subtype 2  DNP  Dinitrophenyl  ER  Endoplasmic reticulum  GFP  Green fluorescent protein  GPCR  G protein–coupled receptor  β-Hex  β-Hexosaminidase  MC  Mast cell  PD  Potential difference  PSA  Passive systemic anaphylaxis  shRNA  Short hairpin RNA  siRNA  Small interfering RNA  SOCE  STIM1  Stromal interacting molecule 1  TBS-T  Tris-buffered saline with 0  1% Tween 20  TER  Transepithelial electrical resistance  TRPC1  Transient receptor potential canonical 1  WT  Wild-type
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