Promoter methylation and clinical significance of GPX3 in esophageal squamous cell carcinoma |
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Authors: | Youbin Lin Ying Zhang Yelong Chen Zhaoyong Liu |
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Institution: | 1. Department of Orthopaedics, First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Shantou, Guangdong 515041, China;2. Department of Pathology, Sun Yat-Sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, China |
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Abstract: | BackgroundGlutathione peroxidase 3 (GPX3) provides critical protection against reactive oxygen species (ROS) in cells. Downregulation of GPX3 may contribute to carcinogenesis of esophageal squamous cell carcinoma (ESCC), but the mechanisms are not clear.Materials and methodsWe examined the differences in gene expression between ESCC and normal esophageal epithelial, by using GEO datasets, and collected 136 ESCC tumors and adjacent normal tissues to confirm our findings. GPX3 expression was measured, at the mRNA and protein levels, by qRT-PCR, Western Blot and immunohistochemistry (IHC).ResultsGPX3 mRNA and protein levels were 3.3-fold higher in normal epithelium compared with case-matched ESCC tissues. There was no significant correlation between clinical parameters and expression levels of GPX3 in ESCC. Promoter methylation of the GPX3 gene correlated with decreased expression.ConclusionDownregulation of GPX3 might be a key factor in the process of ESCC carcinogenesis. |
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Keywords: | Corresponding authors GPX3 ESCC Prognosis IHC Survival |
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