肺癌患者胸腔积液和外周血CD4^＋CD25^＋调节性T细胞与细胞因子表达及其意义的研究

目的：探讨肺癌患者胸腔积液及外周血CD4^＋CD25^＋调节性T细胞及其所分泌的细胞因子的特点及其临床意义。方法：采用流式细胞术和ELISA法检测32例肺癌患者胸腔积液及外周血CD4^＋CD25^＋调节T细胞水平、淋巴细胞亚群和TGF-β、IL-10以及IFN-γ的水平。结果：肺癌患者胸腔积液及外周血CD4^＋CD25^＋调节T细胞水平明显高于正常对照组（t=7．68，P=0．000；t=5．62，P=0．000），且胸腔积液高于外周血（t=2．05 P=0．002）；肺癌患者胸腔积液及外周血CD4、CD4／CD8、CD56阳性细胞比例明显低于正常对照组（t=7．51，P=0．000；t=3．63，P=0．000；t=11．9，P=0．000；t=5．09，P=0．000；t=5．20，P=0．000；t=3．60，P=0．000），且胸腔积液低于外周血（t=3．45，P=0．000；t=5．09，P=0．000；t=2．06，P=0．002），肺癌患者胸腔积液及外周血中TGF-β、IL-10水平高于正常对照组（t=13．38，P=0．000；t=5．2，P=0．000；t=16．14，P=0．000；t=8．07，P=0．000），且胸腔积液高于外周血（t=4．43，P=0．000；t=6．93，P=0．000）；而IFN-γ水平低于正常对照组（t=10．52，P=0．000；t=8．29，P=0．000），且胸腔积液低于外周血（t=2．70，P=0．004）。结论：肺癌患者胸腔积液及外周血CD4^＋CD25^＋调节T细胞水平及其抑制因子的升高，促进了肿瘤的进一步恶化。

Expression and significance of CD4^＋ CD25^＋ regulatory T lymphocytes and cytokines in malignant pleural effusion and peripheral blood of patients with lung cancer

OBJECTIVE： To explore the expression and elinical signficance of CD4^＋ CD25^＋ regulatory T lymphocytes and cytokines in lung cancer. METHODS： The Pleural effusion and peripheral blood were obtained from 32 patients with lung cancer. The percentages of CD4^＋ CD25^＋ cells in total CD4^＋ cells, the expressive rates of CD4, CD56 and the ratio of CD4/CD8 were detected by flow cytometry, the contents of TGF-β, IL-10 and IFN-γ were detected by the enzyme-labeled immunosorbent assay. RESULTS： The percent of CD4^＋ CD25^＋ regulatory T lymphocytes in peripheral blood and effusion of patients with lung cancer was higher than that of healthy volunteers （t=7.58, P=0. 000; t=5.52, P=0. 000）, and the percent of CD4^＋ CD25^＋ regulatory T lymphocytes was higher in the effusion than that in the peripheral blood of the lung cancer patients（t= 2.05, P= 0. 002）, and the expressive rates of CD4, CD4/CD8 and CD56 in peripheral blood and effusion of malignancies were lower than those in healthy volunteers（t = 7.51, P=0.000; t=3.63, P=0.000, t=11. 9, P=0.000; t=5.09, P=0.000; t=5.20, P=0.000; t=3.60, P=0.000）. The contents of TGF-β and IL-10 in peripheral blood of lung cancer were higher than those of healthy volunteers（t = 13.38, P= 0.000; t 5.2, P=0.000; t=16.14,P=0.000; t=8.07, P= 0. 000）,whereas the content of IFN-γ in the lung cancer patients was lower than that of the healthy persons （t = 10.52, P = 0. 000; t = 8.29, P = 0. 000）. CONCLUSION： The relative increase in CD4^＋ CD25^＋ regulatory T cells may be related to the immunosuppression and tumor progression in patients with lung cancer.