丁酸钠对人乳腺癌细胞株MCF-7细胞增殖及p27^kip1蛋白表达影响的研究

目的：通过观察组蛋白去乙酰化酶抑制剂（histon—deacetylase inhibitors，HDACIs）丁酸钠（sodium butyrate，NaB）对人乳腺癌细胞株MCF-7细胞的增殖影响以及p27^kip1蛋白表达改变，探讨NaB调控乳腺癌细胞增殖的分子机制。方法：乳腺癌MCF-7细胞经不同浓度NaB作用后，相差显微镜观察细胞形态变化和细胞增殖情况，流式细胞仪分析细胞周期分布，免疫组化检测p27^kip1蛋白表达。结果：NaB对MCF-7细胞有显著的增殖抑制作用，呈时间剂量依赖性，处理后的MCF-7细胞出现凋亡形态变化；细胞周期阻滞于（G0／G1期，NaB2 mmol／L组G0／G1，期达（62．2±2．2）％，4mmol／L组G0/G1，期达（78．1±3．8）％，空白组G0／C0期达（53．1±2．4）％，P〈0．05；p27蛋白表达水平上调。结论：NaB可抑制乳腺癌细胞的生长，该作用可能与p27蛋白表达增加有关。

Effect of sodium butyrate on proliferation of MCF-7 and expression of p27^kip1 in human breast cancer cell lines

OBJECTIVE： To investigate the effect of sodium butyrate （NaB）on proliferation of human breast cancer cell MCF-7 and expression of p27^kip1. METHODS： A human breast cancer cell line MCF-7 was treated with different concentrations of NaB. A light microscope was used to find out morphological changes in carcinoma cells. The cell cycle status were investigated by flow cytometry, The expression of p27^kip1 was determined by the immunocytochemical assay. RESULTS： NaB significantly inhibited the proliferation of MCF-7 human breast cancer cells in a time- and dosedependent manner. MCF-7 cells treated with NaB was arrested in G0,/G1 phase,（62.2±2.2）% in 2 mmol/L NaB group, （78.1±3.8）% in 4 mmol/L NaB group, and （53.1±2.4）% in control group. Compared with the blank control group, NaB groups were significantly increased, P〈0.05. The expression of p27^kip1 was markedly enhanced after being treated with NaB. CONCLUSION.. NaB can inhibit the growth of breast cancer cell in vitro, which might be associated with the change of p27^kip1 protein expression.