丁酸钠对人乳腺癌细胞株MCF-7细胞增殖及p27^kip1蛋白表达影响的研究 |
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引用本文: | 谢瑞莲,;管晓翔,;陈龙邦,;王靖华,;耿怀成,;朱宝立. 丁酸钠对人乳腺癌细胞株MCF-7细胞增殖及p27^kip1蛋白表达影响的研究[J]. 齐鲁肿瘤杂志, 2008, 0(14): 1041-1044 |
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作者姓名: | 谢瑞莲, 管晓翔, 陈龙邦, 王靖华, 耿怀成, 朱宝立 |
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作者单位: | [1]南京军区南京总医院肿瘤内科,江苏南京210002; [2]江苏省疾病预防控制中心,江苏南京210009 |
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基金项目: | 国家自然科学基金(30470669);江苏省科技厅社会发展项目(3S2005661) |
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摘 要: | 目的:通过观察组蛋白去乙酰化酶抑制剂(histon—deacetylase inhibitors,HDACIs)丁酸钠(sodium butyrate,NaB)对人乳腺癌细胞株MCF-7细胞的增殖影响以及p27^kip1蛋白表达改变,探讨NaB调控乳腺癌细胞增殖的分子机制。方法:乳腺癌MCF-7细胞经不同浓度NaB作用后,相差显微镜观察细胞形态变化和细胞增殖情况,流式细胞仪分析细胞周期分布,免疫组化检测p27^kip1蛋白表达。结果:NaB对MCF-7细胞有显著的增殖抑制作用,呈时间剂量依赖性,处理后的MCF-7细胞出现凋亡形态变化;细胞周期阻滞于(G0/G1期,NaB2 mmol/L组G0/G1,期达(62.2±2.2)%,4mmol/L组G0/G1,期达(78.1±3.8)%,空白组G0/C0期达(53.1±2.4)%,P〈0.05;p27蛋白表达水平上调。结论:NaB可抑制乳腺癌细胞的生长,该作用可能与p27蛋白表达增加有关。
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关 键 词: | 丁酸类/药理学 乳腺肿瘤/病理学 细胞周期 P27蛋白 |
Effect of sodium butyrate on proliferation of MCF-7 and expression of p27^kip1 in human breast cancer cell lines |
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Affiliation: | XIE Rui-lian , GUAN Xiao-xiang , CHEN Long-bang , WANG Jing-hua , GENG Huai-cheng , ZHU Bao-li( 1. Department of Oncology, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002, P. R. China 2. Jiangsu Center for Disease Control and Prevention, Nanjing 210009, P. R. China) |
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Abstract: | OBJECTIVE: To investigate the effect of sodium butyrate (NaB)on proliferation of human breast cancer cell MCF-7 and expression of p27^kip1. METHODS: A human breast cancer cell line MCF-7 was treated with different concentrations of NaB. A light microscope was used to find out morphological changes in carcinoma cells. The cell cycle status were investigated by flow cytometry, The expression of p27^kip1 was determined by the immunocytochemical assay. RESULTS: NaB significantly inhibited the proliferation of MCF-7 human breast cancer cells in a time- and dosedependent manner. MCF-7 cells treated with NaB was arrested in G0,/G1 phase,(62.2±2.2)% in 2 mmol/L NaB group, (78.1±3.8)% in 4 mmol/L NaB group, and (53.1±2.4)% in control group. Compared with the blank control group, NaB groups were significantly increased, P〈0.05. The expression of p27^kip1 was markedly enhanced after being treated with NaB. CONCLUSION.. NaB can inhibit the growth of breast cancer cell in vitro, which might be associated with the change of p27^kip1 protein expression. |
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Keywords: | butyric acids/pharmacology breast neoplasms/pathology cell cycle p27^kip1 protein |
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