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Evaluation of a semi-automated 24-hour commercial system for identification ofEnterobacteriaceae and other gram-negative bacteria
Authors:D. Monnet  D. Lafay  M. Desmonceaux  J. M. Boeufgras  F. Allard  J. Freney
Affiliation:(1) Laboratoire de Microbiologie, Faculté de Médecine Lyon-Nord, Lyon, France;(2) DERBA, EA 16SS Laboratoire de Bactériologie, Faculté de Médecine Alexis-Carrel, and Hôpital Edouard Herriot, Place d'Arsonval, 69437 Lyon Cedex 03, France;(3) Laboratoire de Recherche, bioMérieux, La Balme-les-Grottes, France
Abstract:A semi-automated commercial system (ID 32 E, bioMérieux) for 24-hour identification ofEnterobacteriaceae and other gram-negative fermentative and nonfermentative bacteria encountered in diagnostic microbiology was evaluated. Overall, the system correctly identified 506 (91.5 %) of the 553 strains tested, 94 (17.0 %) strains requiring additional tests for complete identification. Six (1.1 %) strains were misidentified and 33 (6.0 %) strains were not identified. Eight (1.4 %) strains were not present in the database and were misidentified or not identified. The system is a suitable alternative to existing systems for the identification ofEnterobacteriaceae and other gram-negative bacteria frequently encountered in clinical samples.
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