抗EB病毒膜抗原单克隆抗体的制备及鉴定

本文将sp_2/o细胞与经脾内注射用0.2mMH2O2激活的B95—8全细胞免疫的BALB/C小鼠脾细胞,用50％PEG4000进行融合,其平均融合率为51.79%,抗体分泌为43.48％。经亚克隆后获得一株能持续分泌抗EB病毒脱抗原的单克隆抗体(以下简称抗EBV—MA单抗)的杂交瘤细胞—B3B7,该细胞的染色体数为111.7±4.76。经ELISA法,间接免疫酶法和间接免疫荧光法证实该单抗不能与EBV—MA(一)的人Rajì细胞发生特异性免疫反应,只能与EBV—MA(+)的人P3HR—1和绒猴的B95—8细胞的反应,而且与H_2O_2激活的B95—8和P_3HX—1细胞的反应强度非常显著地高于与未经H_2O_2激活的B95—8和P_3HR—1细胞的反应强度(P<0.001),同时亦证实特异性荧光主要集中在EBV—MA(+)细胞的细胞膜上,从而表明B_3B_7确实是能分泌抗EBV—MA单抗的杂交瘤细胞。

PRODUCTION AND INVESTIGATION OF MONOCLONAR ANTIBODIES TO EPSTEIN BARR VIRUS ANTIGEN

4 female BAcB/C mice were timmunized hy EBV—MA (+) B95 —8 cells aetivated with 0.2mMH_2O_2 The immunized mouse spflen cells and sp 2/0 myeloma cells were hroughtl together and fused hy 50% PEG4000 Ahyhridoma cell line—B_3B_7, whose chromoaoma numher was 111.7±64.76, was gat, The McAh whieh the hyhridoma cell line ralaed oney hound to EBV —MA (+) Catton—top marmoset B95—8cell line and human PHR—1 cell line, but not to EBV—AM (—) human Raji cell line, Morever, its reactive sttrength withB95—8 and P_3HR—1 cell line activated byH_2O_2 increased very markedly These experimental resuets suggested the McAb was of speciqieity aganist EBV—MA indeed, The EBV—MA McAb maybe usecd to study the diagnosis, theray and prevention of NPC