应用趋化因子受体标记对系统性红斑狼疮患者Th细胸亚群改变及其意义的初步研究

目的探讨系统性红斑狼疮(SLE)活动期患者Th红胞亚群平衡的改变及其意义.方法以趋化因子受体CCR5和CCR3为Th红胞亚群表面标记,利用三色流式细胞分析法对SLE患者外周血单个核细胞(PBMCs)中CD4+T细胞亚群平衡变化进行分析.结果活动期SLE患者外周血CC4+CCR5+CCR3-细胞百分比与正常人比较差异无显著性(P>0.05).CD4+CCR5-CCR3+和(或)CD4+CCR5+CCR3-细胞百分比异常升高者出现组织脏器损伤的概率较高.肾上腺糖皮质激素治疗可使异常升高的CD4+CCR5-CCR3+和(或)CD4+CCR5+CCR3-细胞百分比有所降低.结论多数活动期SLE患者体内存在Th2细胞优势,Th2和(或)Th1细胞的改变与组织脏器损伤有一定的相关性.

Significance of chemokine receptors CCR3 and CCR5 on T helper cells of systemic lupus erythematosus patients

Objective To study the significance of T helper cells in patients with active systemic lupus erythematosus.Method The levels of CD4 + CCR5 + CCR3 - and CD4 + CCR5 - CCR3 + were measured by three-color cytometry.Results Compared with healthy controls,the percentage of CD4 + CCR5 + CCR3 - cells had no significant change,while the percentage of CD4 + CCR5 - CCR3 + cells was significantly increased in SLE patients.The ratio of CD4 + CCR5 + CCR3 - /CD4 + CCR5 - CCR3 + was significantly lower than control group.Patients with abnormaly high level of CD4 + CCR5 - CCR3 + cells and/or CD4 + CCR5 + CCR3 - cells,usually had more organ damages.The glucocorti-coid therapy could partly reverse the imbalance.Conclusion There is significant predominance of Th2and/or Th1cells in some patients with active SLE.It may correlate to organ damages.