肺鳞癌和肺腺癌中抑癌基因缺失的比较研究

目的 比较中国人肺鳞癌和肺腺癌中抑癌基因丢失的情况,分析抑癌基因在肺癌发生中的不同作用。方法 选取位于13个抑癌基因侧翼（与基因紧密连锁）或位于基因内含子区的22个微卫星位点,对28例肺鳞癌和13例肺腺癌标本进行杂合缺失分析。结果 FHIT、p53、INFNA、VHL和p16基因在肺鳞癌和肺腺癌中均有高频率的杂合缺失,而PRLTS、PTEN和p57基因的杂合缺失率在肺鳞癌和肺腺癌中有明显差异。结论 在肺鳞癌和肺腺癌发病过程中可能有不同的抑癌基因失活,其中PRLTS可能对肺腺癌的发生有重要作用。

Comparison of tumor suppressor gene deletion between squamous cell carcinoma and adenocarcinoma lung cancer in Chinese

Objective To prove the hypothesis that there may be different tumor suppressor genes (TSGs) involved in genesis of squamous cell carcinoma (SCC) and adenocarcinoma (ADC) in non small cell lung cancer (NSCLC) in the Chinese.Methods Twenty two microsatellite markers flanking to or within 13 identified or candidate TSGs were used to detect loss of heterozygosity (LOH) in these regions in the specimens of 28 SCC patients and 13 ADC patients.Results Our data demonstrated frequent allelic loss of FHIT, p53, IFNA, VHL and p16 both in SCC and ADC. Three genes, PRLTS, PTEN and p57, showed statistically significant differences in the LOH frequency between SCC and ADC.Conclusion The comparison of LOH frequencies between SCC and ADC indicates possible correlation between specific tumor suppressor genes and each type of lung cancer. The results also suggest that more attention should be paid to the PRLTS gene, which may play an important role in the development of ADC. [