SOX2通过PI3K/AKT通路调控口腔鳞癌细胞迁徙的机制研究

目的:研究过表达SOX2通过PI3K/AKT通路促进口腔鳞癌(oral squamous cell carcinoma,OSCC)细胞迁移及上皮间质转化(epithelial mesenchymal transition,EMT)的作用及机制.方法:收集OSCC组织及正常口腔黏膜组织,培养OSCC细胞株Tca83、SC...

Mechanism of overexpression of SOX2 in regulating migration and EMT of oral squamous cell carcinoma cells through PI3K/AKT pathway

Objective:To investigate the effect and mechanism of overexpression of SOX2 on the migration and epithelial mesenchymal transition(EMT)of oral squamous cell carcinoma(OSCC)cells through PI3 K/AKT pathway.Methods:OSCC tissues and normal oral mucosa tissues were collected,OSCC cell lines Tca83,SCC25,HN4 and normal oral epithelial cell line FM were cultured,and the mRNA expression level of SOX2 was detected.Tca83 cells were randomly divided into negative control(NC)group transfected with NC plasmid,SOX2 group transfected with SOX2 plasmid,placebo+NC group transfected with NC plasmid and added with placebo,placebo+SOX2 group transfected with SOX2 plasmid and added with placebo,LY294002+SOX2 group transfected with SOX2 plasmid and added with PI3 K inhibitor LY294002.Then the cell migration,the expression of vimentin,E-cadherin,p-PI3 K and p-AKT were detected.Results:The mRNA expression of SOX2 in OSCC was higher than that in normal oral mucosa,and the mRNA expression of SOX2 in Tca83,SCC25 and HN4 cells was higher than that in FM cells,and the increase of SOX2 expression in Tca83 cells was the weakest.Compared with NC group,the migration enhanced,the expression of vimentin,p-PI3 K and p-AKT increased,and the expression of E-cadherin decreased in SOX2 group.Compared with placebo+SOX2 group,the migration weakened,the expression of vimentin,p-PI3 K and p-AKT decreased,and the expression of E-cadherin increased in LY294002+SOX2 group.Conclusion:The expression of SOX2 increases in OSCC and overexpression of SOX2 promotes migration and EMT of OSCC cells by activating PI3 K/AKT pathway.